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Pflügers Archiv

, Volume 394, Issue 4, pp 347–354 | Cite as

Actions of prostaglandins and indomethacin on the electrical and mechanical properties of smooth muscle cells of the guinea-pig ileocecal junction

  • Masayuki Kubota
  • Yushi Ito
  • Mariko Domae
Excitable Tissues and Central Nervous Physiology

Abstract

The effects of prostaglandins (PGs) and indomethacin on the mechanical and membrane properties of the smooth muscle cells of the guinea-pig ileocecal junction were studied using microelectrode and tension recording techniques and radioimmunoassay to determine levels of PGs. In the guinea-pig ileocecal junction, we found two distinct cell populations-cells with, and without spontaneous electrical and mechanical activities. PGs (PGE1, PGE2, PGF) in low concentrations suppressed spontaneous mechanical activity. Correspondingly, PGs (>10−7 M) suppressed both spontaneously generated spikes and evoked spikes, presumably due to an increase in the threshold for generation of action potential. On the contrary, indomethacin evoked rhythmic spontaneous contractions in the mechanically quiescent muscle preparations and reduced the PGE and PGF content of the muscle. Spontaneous spike discharges occurred during the indomethacin-induced contractions. The spontaneous electrical and mechanical activities induced by indomethacin were suppressed by PGs, in low concentrations. These results indicate that under physiological conditions, the endogenous PGs in the muscle may play an important role in regulating muscle tone as well as the membrane properties, thereby contributing to the regulation of motility of the intestine and possibly the sphincter.

Key words

PGs Indomethacin Smooth muscle Ileocecal junction 

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References

  1. Bennett A, Fleshler B (1970) Prostaglandins and the gastrointestinal tract. Gastroenterology 59:790–800Google Scholar
  2. Bennett A, Friedmann CA, Vane JR (1967) Release of prostaglandin E1 from the rat stomach. Nature 216:873–876Google Scholar
  3. Bennett A, Eley KG, Scholes GB (1968) Effects of prostaglandin E1 and E2 on human, guinea-pig and rat isolated small intestine. Br J Pharmacol 34:630–638Google Scholar
  4. Botting JH, Salzmann R (1974) The effect of indomethacin on the release of prostaglandin E2 and acetylcholine from guinea-pig isolated ileum at rest and during field stimulation. Br J Pharmacol 50:119–124Google Scholar
  5. Dajani EZ, Roge EAW, Bertermann RE (1975) Effects of prostaglandins, diphenoxylate and morphine on intestinal motility in vivo. Eur J Pharmacol 34:105–113Google Scholar
  6. Ferreira SH, Herman A, Vane JR (1972) Prostaglandin generation maintains the smooth muscle tone of the rabbit isolated jejunum. Br J Pharmacol 44:328P-329PGoogle Scholar
  7. Folch J, Lees M, Sloane-Stanley GH (1957) A simple method for the isolation and purification of total lipids from animal tissues. J Biol Chem 226:497–509Google Scholar
  8. Ito Y, Tajima K (1979) An electrophysiological analysis of the actions of prostaglandin on neuromuscular transmission in the guinea-pig vas deferens. J Physiol 297:521–537Google Scholar
  9. Ito Y, Tajima K (1981a) Actions of indomethacin and prostaglandins on neuro-effector transmission in the dog trachea. J Physiol 319:379–392Google Scholar
  10. Ito Y, Tajima K (1981b) Spontaneous activity in the trachea of dogs treated with indomethacin: an experimental model for aspirin-related asthma. Br J Pharmacol 73:563–571Google Scholar
  11. Jaffe BM, Behrman HR (1974) Prostaglandin E., A and F. In: Jaffe BM, Behrman HR (eds) Methods of hormone radioimmunoassay. Academic Press, New York, p 19Google Scholar
  12. Jouvenaz GH, Nugteren DH, Beerthuis RK, Van Dorp DA (1970) A sensitive method for the determination of prostaglandins by gas chromatography with electron-capture detection. Biochim Biophys Acta 202:231–233Google Scholar
  13. Kubota M (1982) Electrical and mechanical properties and neuro-effector transmission in the smooth muscle layer of the guinea-pig ileocecal junction. Pflügers Arch 394:355–361Google Scholar
  14. Kuriyama H, Suzuki H (1975) On the electrical and mechanical properties of the longitudinal and circular muscles of the guinea-pig ileum. INSERM, 16-18 juillet 50:197–214Google Scholar
  15. Kuriyama H, Makita Y (1982) Modulation of neuromuscular transmission by endogenous and exogenous prostaglandins in the guineapig mesenteric artery. J Physiol (in press)Google Scholar
  16. Sanders KM (1978) Endogeneous prostaglandin E and contractile activity of isolated ileal smooth muscle. Am J Physiol 3:E209–212Google Scholar
  17. Sanders KM, Ross G (1978) Effects of endogeneous prostaglandin E on intestinal motility. Am J Physiol 3:E204-E208Google Scholar
  18. Shehadeh Z, Price WE, Jacobson ED (1969) Effects of vasoactive agents on intestinal blood flow and motility in the dog. Am J Physiol 216:386–392Google Scholar
  19. Türker RK, Onur R (1971) Effects of prostaglandin E1 on intestinal motility of the cat. Arch Intern Physiol Biochem 79:535–543Google Scholar
  20. Willis AI, Davison P, Ramwell PW (1974) Inhibition of intestinal tone, motility and prostaglandin biosynthesis by 5,8,11,14-eicosatetraynoic acid. Prostaglandins 5:355–368Google Scholar

Copyright information

© Springer-Verlag 1982

Authors and Affiliations

  • Masayuki Kubota
    • 1
  • Yushi Ito
    • 1
  • Mariko Domae
    • 2
  1. 1.Department of Pharmacology, Faculty of MedicineKyushu UniversityFukuokaJapan
  2. 2.Research Institute for Diseases of the Chest, Faculty of MedicineKyushu UniversityFukuokaJapan

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