Pflügers Archiv

, Volume 394, Issue 4, pp 329–332 | Cite as

Relation between membrane potential and lactate in gastrocnemius and soleus muscle of the cat during tourniquet ischemia and postischemic reflow

  • Eva Jennische
Excitable Tissues and Central Nervous Physiology


The relation between the lactate content and the membrane potential was investigated in the gastrocnemius and soleus muscle of the cat during and after a 4 h period of ischemia. The skeletal muscle content of ATP and glucose was also measured. No change occurred in the ATP content of the gastrocnemius muscle during the period of ischemia, whereas in the soleus a 40% reduction of ATP occurred. The glucose content decreased during ischemia and increased above initial values after reflow in both muscles. The lactate content increased, and the membrane potential decreased linearly in both muscles during the ischemic period. The final lactate accumulation was higher and the decrease in membrane potential was less in the gastrocnemius than in the soleus. After release of the tourniquet both variables returned to normal or near normal values within 1.5 h in both muscles. A significant correlation was found between the lactate content and the membrane potential in both muscles during the entire experimental period. It is suggested that the depolarisation occurring in skeletal muscle during hypoxia is partly caused by changes in intracellular pH.

Key words

Ischemia Lactate Membrane potential Skeletal muscle 


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  1. Aickin CC, Thomas RC (1977) Micro-electrode measurement of the intracellular pH and buffering power of mouse soleus muscle fibres. J Physiol 267:791–810Google Scholar
  2. Andersson J, Eklöf B, Neglén P, Thomson D (1979) Metabolic changes in blood and skeletal muscle in reconstructive aortic surgery. Ann Surg 189:283–289Google Scholar
  3. Balasubramanian V, McNamara DB, Singh JN, Dhalla NS (1973) Biochemical basis of heart function. X. Reduction in the Na+−K+-stimulated ATPase activity in failing rat heart due to hypoxia. Can J Physiol Pharmacol 51:504–510Google Scholar
  4. Beatty CH, Peterson RD, Bocek RM (1963) Metabolism of red and white muscle fiber groups. Am J Physiol 204:939–942Google Scholar
  5. Clausen T, Flatman JA (1977) The effect of cathecholamines on Na−K transport and membrane potential in rat soleus muscle. J Physiol (Lond) 270:383–414Google Scholar
  6. Davey CL (1960) The significance of carnosine and anserine in striated skeletal muscle. Arch Biochem Biophys 89:303–308Google Scholar
  7. DeWall RA, Vasko KA, Stanley EL, Kezdi P (1971) Response of the ischemic myocardium to allopurinol. Am Heart J 82:362–370Google Scholar
  8. Dubowitz V, Pearse AGE (1960) Reciprocal relationship of phosphorylase and oxidative enzymes in skeletal muscle. Nature (Lond) 185:701–702Google Scholar
  9. Enger EA, Jennische E, Medegård A, Haljamäe H (1978) Cellular restitution after 3 h of complete tourniquet ischemia. Eur Surg Res 10:230–239Google Scholar
  10. Folkow B, Halicka HD (1968) A comparison between “red” and “white” muscle with respect to blood supply, capillary surface areas and oxygen uptake during rest and exercise. Microvasc Res 1:1–14Google Scholar
  11. Haljamäe H, Enger E (1975) Human skeletal muscle energy metabolism during and after complete tourniquet ischemia. Ann Surg 182:9–14Google Scholar
  12. Honig CR, Frierson JL, Nelson CN (1971) O2 transport and VO2 in resting muscle: significance for tissue-capillary exchange. Am J Physiol 220:357–363Google Scholar
  13. Hudlicka O (1973) Anatomy and histology of muscle circulation. Muscle blood flow. Swets & Zeitlinger B. V., Amsterdam, pp 3–26Google Scholar
  14. Jennische E, Enger E, Medegård A, Appelgren L, Haljamäe H (1978) Correlation between tissue pH, cellular transmembrane potentials, and cellular energy metabolism during shock and during ischemia. Circ Shock 5:251–260Google Scholar
  15. Jennische E, Amundson B, Haljamäe H (1979) Metabolic responses in feline ”red” and “white” skeletal muscle to shock and ischemia. Acta Physiol Scand 106:39–45Google Scholar
  16. Karpf M, Stock W, Gebert E, Kruse-Jarres JD, Zimmermann W (1974) Stoffwechselveränderungen und Restitution nach temporärer Tourniquet Ischämie beim Menschen. Langenbecks Arch Chir 335:307–311Google Scholar
  17. Kipnis DM, Helmreich E, Cori CF (1959) Studies of tissue permeability. IV. The distribution of glucose between plasma and muscle. J Biol Chem 234:165–170Google Scholar
  18. Lambotte L (1977) Effect of anoxia and ATP depletion on the membrane potential and permeability of dog liver. J Physiol (Lond) 269:53–76Google Scholar
  19. Lowry OH, Rosebrough NJ, Farr AL, Randall RJ (1951) Protein measurement with the Folin phenol reagent. J Biol Chem 193:265–275Google Scholar
  20. Lykkeboe G, Johansen K (1975) Comparative aspects of buffering capacity in muscle. Respir Physiol 25:353–361Google Scholar
  21. Miller SH, Price G, Buck D, Neeley J, Kennedy TJ, Gram WP, Davis TS (1979) Effects of tourniquet ischemia and postischemic edema on muscle metabolism. J Hand Surg 4:547–555Google Scholar
  22. Maxwell LC, Barclay JK, Mohrman DE, Faulkner JA (1977) Physiological characteristics of skeletal muscle of dogs and cats. Am J Physiol 233:C14-C18Google Scholar
  23. Peter JB, Barnard J, Edgerton R, Gillespie CA, Stempel KE (1972) Metabolic profiles of three fibre types of skeletal muscle in guinea pigs and rabbits. Biochemistry 11:2627–2633Google Scholar
  24. Roos A, Boron WF (1981) Intracellular pH. Physiol Rev 61:296–434Google Scholar
  25. Sahlin K, Harris RC, Nylind B, Hultman E (1976) Lactate content and pH in muscle samples obtained after dynamic exercise. Pflügers Arch 367:143–149Google Scholar
  26. Stock W, Isselhard W (1971) Postischemic hyperemia and metabolic changes after long-lasting blood flow interruption of a dog extremity. Vasc Surg 6:255–262Google Scholar
  27. Stock W, Bohn HJ, Isselhard W (1973) Die Restitution des Energiestoffwechsels der Skelettmuskulatur der Ratte nach langdauernder Ischämie. Res Exp Med 159:306–320Google Scholar
  28. Zierler K, Rogus EM (1981) Effects of peptide hormones and adrenergic agents on membrane potentials of target cells. Feder Proc 40:121–124Google Scholar

Copyright information

© Springer-Verlag 1982

Authors and Affiliations

  • Eva Jennische
    • 1
  1. 1.Department of HistologyUniversity of GöteborgGöteborgSweden

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