Some technical improvements for the voltage clamp with the double sucrose gap
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A new double sucrose gap chamber using electrodes of high stability and low resistance is described. The size of each sucrose gap and the central node can be modified independently so that this chamber is suitable for research on multicellular or unicellular excitable preparation under voltage clamp conditions. The shape of the central channel is made so as to reduce the series resistance between the preparation and the recording electrode to a minimum. A window made in the floor of the chamber allows to observe the preparation under a microscope.
Key wordsDouble sucrose gap technique Electrodes Voltage clamp
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- Julian, F. J., Moore, J. W., Goldman, D. E.: Current-voltage relations in the lobster giant membrane under voltage clamp conditions. J. gen. Physiol.45, 1217–1238 (1962)Google Scholar
- Léoty, C., Poindessault, J. P.: Effects and compensation of the series resistance in voltage clamp experiments using double sucrose gap technique. J. Physiol. (Lond.)239, 108–109P (1974)Google Scholar
- Léoty, C., Raymond, G.: Mechanical activity and ionic currents in frog atrial trabeculae. Pflügers Arch.334, 114–128 (1972)Google Scholar
- Poindessault, J. P., Duval, A., Léoty, C.: Voltage clamp with double sucrose gap technique; external series resistance compensation. Biophys. J.16, 105–120 (1976)Google Scholar
- Rougier, O., Vassort, G., Stämpfli, R.: Voltage clamp experiments on frog atrial heart muscle fibres with the double sucrose gap technique. Pflügers Arch. ges. Physiol.301, 91–108 (1968)Google Scholar
- Stämpfli, R.: A new method for measuring membrane potentials with external electrodes. Experientia (Basel)10, 508–509 (1954)Google Scholar
- Tarr, M., Trank, J.: Equivalent circuit of frog atrial tissue as determined by voltage clamp: unclamp experiments. J. gen. Physiol.58, 511–522 (1971)Google Scholar