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Pflügers Archiv

, Volume 365, Issue 1, pp 61–68 | Cite as

Metabolic control of respiratory neuronal activity and the accompanying changes in breathing movements of the rabbit

II. Manipulation of expiratory and inspiratory-expiratory neurons
  • G. Böhmer
  • R. A. Chaplain
  • M. Fallert
Article

Summary

Expiratory-related neurons have been classified according to their phase relation within the respiratory cycle, their response to lung distension and collapse (α- and β-type), and to hyperventilation (tonic firing denoted by “+”, cessation of activity by “−”). The dorsal surface of the medulla oblongata was superfused with a metabolite-containing CSF solution and the activity of expiratory (E) and inspiratory-expiratory (IE) neurons was extracellularly recorded. The neuronal sub-types established by their functional behaviour could equally be distinguished by their differential response to one or several metabolites. In contrast to inspiratory (I) neurons, E α , E + β , E β and IE β neurons are inhibited by 3.5 mM AMP, but are activated by 10 mM citrate (with the exception of E + β units). Furthermore I cells are activated by ATP, while Eα and Eβ units become inhibited. Vagotomy in some instances affected the response of some IEβ units. An increase in spike density of IE + β and E α cells in paralleled by a reduction of both the respiratory rate and the tidal volume, while a lower spike density in IE + β , IE β and E α units is accompanied by increases in respiratory rate and tidal volume. In the case of E + β and E β cells lower activity is associated with an increased tidal volume. No metabolite-induced changes could be obtained with cardiovascular or unspecific reticular neurons.

Key words

Bulbar respiratory centre Expiratory neurons Metabolic modifiers of neuronal activity Tidal volume Respiratory rate Stereotaxic mapping of respiratory neurons 

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References

  1. Chaplain, R. A.: Metabolic regulations of the rhythmic activity in pacemaker neurons. II. Metabolically induced conversion of beating to bursting activity in Aplysia neurons. Brain Res.106, 307–319 (1976)Google Scholar
  2. Chaplain, R. A., Dinse, H. R. O., Fallert, M.: Metabolic control of respiratory neuronal activity and the accompanying changes in breathing movements of the rabbit. I. Manipulation of inspiratory and expiratory-inspiratory neurons. Pflügers Arch.365, 49–59 (1976)Google Scholar
  3. Chaplain, R. A., Krämer, G..: Metabolic regulations of the rhythmic activity in pacemaker neurons. I. Frequency-setting of beating Aplysia pacemakers by modifiers of phosphofructokinase and fructose-1,6-diphosphatase. Pflügers Arch.359, (Suppl.), R 83 (1975)Google Scholar
  4. Cohen, M. I.: Discharge patterns of brain stem respiratory neurons in relation to carbon dioxide tension. J. Neurophysiol.31, 142–165 (1968)Google Scholar
  5. Cohen, M. I.: The genesis of respiratory rhythmicity In Central rhythmic and regulation, circulation, respiration, extrapyramidal motor system (W. Umbach and H. P. Koepchen, eds.), pp. 15–35. Stuttgart: Hippokrates-Verlag 1974Google Scholar
  6. Fallert, M., Baum, K.: The bulbar respiratory centre in the rabbit. II. Responses of respiratory neurons to intermittent electrical bulbar stimulation during in-or expiration. Pflügers Arch.364, 269–284 (1976)Google Scholar
  7. Frazier, W. T., Kandel, E. R., Kupfermann, I., Waziri, R., Coggeshall, R. E.: Morphological and functional properties of identified neurons in the abdominal ganglion of Aplysia californica. J. Neurophysiol.30, 1288–1351. (1967)Google Scholar
  8. Koepchen, H. P., Langhorst, P., Seller, H.: The problem of identification of autonomic neurons in the lower brain stem. Brain Res.87, 375–393 (1975)Google Scholar
  9. Winkler, C. and Potter, A.: An anatomical guide to experimental researches on the rabbit's brain. A series of 40 frontal sections. Amsterdam: Versluys 1911Google Scholar

Copyright information

© Springer-Verlag 1976

Authors and Affiliations

  • G. Böhmer
    • 1
  • R. A. Chaplain
    • 1
  • M. Fallert
    • 1
  1. 1.Physiologisches Institut der Universität MainzMainzGermany

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