Pflügers Archiv - European Journal of Physiology

, Volume 413, Issue 3, pp 319–321 | Cite as

GTP-binding proteins mediate acetylcholine inhibition of voltage dependent calcium channels in hippocampal neurons

  • Mauro Toselli
  • Hans Dieter Lux
Excitable Tissues Cand Central Nervous Physiology Short Communication


Acetylcholine (Ach) was tested for its effect on calcium currents in primary cultures of embryonic rat hippocampal neurones. 10 μM Ach reversibly depressed the high voltage activated (HVA) Ca current and the effect was blocked by atropine. When GTP binding proteins (G-proteins) were irreversibly activated by intracellularly perfusing the neurons with GTP[ S], one short-lasting application of Ach permanently inhibited Ca currents. Alternatively, the transmitter was inactive when the cells were preincubated with pertussis toxin, which inactivates some G-proteins by catalyzing ADP ribosylation. Our results suggest that a G-protein mediates the inhibitory modulation of the HVA Ca current by muscarinic agonists.

Key words

Ca channels Acetylcholine G-proteins Hippocampus 


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Copyright information

© Springer-Verlag 1989

Authors and Affiliations

  • Mauro Toselli
    • 1
  • Hans Dieter Lux
    • 1
  1. 1.Abteilung NeurophysiologieMax-Planck-Institut für PsychiatriePlaneggGermany

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