Advertisement

Pflügers Archiv - European Journal of Physiology

, Volume 413, Issue 3, pp 299–302 | Cite as

Prostaglandins and neurotransmission at the guinea pig and rabbit urinary bladder

  • Kate E. Creed
  • Stephen M. Callahan
Excitable Tissues Cand Central Nervous Physiology

Abstract

High concentrations of prostaglandins (PGE1, PGE2, or PGE) (2×10−6 M) produced a slow contraction of longitudinal strips of detrusor muscle taken from the bladders of guinea pigs and rabbits. At a lower concentration (10−6 M) prostaglandins enhanced contractions produced by field stimulation of nerves in guinea pig but not rabbit strips. The contractions were not affected by indomethacin. Contractions of guinea pig strips in response to acetylcholine at 10−4 M were enhanced by prostaglandins and unaffected by indomethacin. Membrane potentials of smooth muscle cells recorded with micro electrodes, were unchanged up to 10−6 M PGE2. Above this the cells were depolarized with an increase in frequency of spontaneous action potentials. Synchronous recording of electrical and mechanical activity with the double sucrose gap indicated a decrease in amplitude of the evoked excitatory junction potential and action potential even when the contraction was enhanced in the presence of PGE2. Responses to repeated stimulation at 10 Hz for 1 min were progressively depressed. This trend was slightly reduced by PGE2 but unaffected by indomethacin. It is concluded that prostaglandins are not normally released by the nerves to the urinary bladder but are able to facilitate contraction in the guinea pig. This effect is probably on the excitatory-contraction coupling, possibly by mobilizing Ca2+. Some modification of transmitter release by the nerves may also occur.

Key words

Prostaglandins Indomethacin Urinary bladder Electrical activity Mechanical activity Neurotransmission 

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. Ambache N, Zar AB (1970) Non-cholinergic transmission by postganglionic motor neurones in the mammalian bladder. J Physiol 210:761–783CrossRefPubMedPubMedCentralGoogle Scholar
  2. Anderson GF (1982) Evidence for a prostaglandin link in the purinergic activation of rabbit bladder smooth muscle. J Pharmacol Exp Ther 220:347–352PubMedGoogle Scholar
  3. Andersson KE, Forman A (1978) Effects of prostaglandins on the smooth muscle of the urinary tract. Acta Pharmacol Toxicol (Copenh) 43 (suppl) 90–95Google Scholar
  4. Choo LK, Mitchelson F (1977) The role of prostaglandins in the excitatory innervation of the rat urinary bladder. Prostaglandins 13:917–926CrossRefPubMedGoogle Scholar
  5. Creed KE (1971) Effects of ions and drugs on the smooth muscle cell membrane of the guinea pig urinary bladder. Pflügers Arch 326:127–141CrossRefPubMedGoogle Scholar
  6. Creed KE, Ishikawa S, Ito Y (1983) Electrical and mechanical activity recorded from rabbit urinary bladder in response to nerve stimulation. J Physiol 338:149–164CrossRefPubMedPubMedCentralGoogle Scholar
  7. Dean DW, Downie JW (1978) Interaction of prostaglandins and adenosine 5′-triphosphate in the non-cholinergic neurotransmission in rabbit detrusor. Prostaglandins 16:245–251CrossRefPubMedGoogle Scholar
  8. Hedqvist P (1969) Antagonism between prostaglandin E2 on adrenaline release from the isolated cat spleen. Acta Physiol Scand 75:511–512CrossRefPubMedGoogle Scholar
  9. Hills NH (1976) Prostaglandins and tone in isolated strips of mammalian bladder. Br J Pharmacol 57:464PGoogle Scholar
  10. Ito Y, Tajima K (1979) An electrophysiological analysis of the actions of prostaglandins on neuromuscular transmission in the guinea pig vas deferens. J Physiol 297:521–537CrossRefPubMedPubMedCentralGoogle Scholar
  11. Ito Y, Tajima K (1981) Actions of indomethacin and prostaglandins on neuro-effector transmission in the dog trachea. J Physiol 319:379–392CrossRefPubMedPubMedCentralGoogle Scholar
  12. Johns A, Paton DM (1977) Effects of indomethacin on atropine resistant transmission in rabbit and monkey urinary bladder. Prostaglandins 13:245–254CrossRefPubMedGoogle Scholar
  13. Klockner U, Isenberg G (1985) Calcium currents of cesium loaded isolated smooth muscle cells (urinary bladder of the guinea pig). Pflügers Arch 405:340–348CrossRefPubMedGoogle Scholar
  14. Langley JN, Anderson HK (1895) Innervation of the pelvic and adjoining viscera. II. The bladder. J Physiol 19:71–84CrossRefPubMedPubMedCentralGoogle Scholar
  15. Moore PK (1985) Prostanoids: Pharmacological, physiological and clinical relevance. Cambridge University Press, CambridgeGoogle Scholar
  16. Ursillo RC, Clark BB (1956) The actions of atropine on the urinary bladder of the dog. J Pharmacol Exp Ther 118:338–347PubMedGoogle Scholar

Copyright information

© Springer-Verlag 1989

Authors and Affiliations

  • Kate E. Creed
    • 1
  • Stephen M. Callahan
    • 1
  1. 1.School of Veterinary StudiesMurdoch UniversityMurdochAustralia

Personalised recommendations