Advertisement

Pflügers Archiv - European Journal of Physiology

, Volume 413, Issue 3, pp 217–224 | Cite as

Transport and utilization of α-ketoglutarate by the rat kidney in vivo

  • M. Martin
  • B. Ferrier
  • G. Baverel
Transport Processes, Metabolism and Bendocrinology; Kidney, Gastrointestinal Tract, and Exocrine Glands

Abstract

In order to establish the characteristics of net renal transport and utilization of α-ketoglutarate (α-KG) in the rat, we have precisely quantified the renal blood flow, the urinary flow and the rates of α-KG delivery, filtration, reabsorption or secretion, excretion, uptake or production by an in vivo rat kidney preparation. In normal rats, α-KG uptake was higher than α-KG reabsorption at both endogenous and elevated plasma α-KG concentrations; thus, a net peritubular transport, which was the main supplier of α-KG to the renal cells, took place. Saturation of reabsorption and peritubular transport of α-KG occurred at blood α-KG concentrations about 30 and 150 times above normal, respectively. Acute metabolic acidosis was found to have no effect on renal handling of α-KG. At endogenous plasma α-KG concentrations, alkalosis converted net renal uptake into net renal production of α-KG resulting in addition of α-KG by the renal cells both to blood and to the luminal fluid. Elevation of blood α-KG concentration restored the renal uptake of α-KG. This uptake, which was entirely accounted for by the peritubular transport of α-KG, reached a maximum which was lower than that observed in normal and acidotic rats.

Key words

Rat Kidney Uptake Transport α-Ketoglutarate Luminal Basolateral Production 

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. Arendshorst WJ, Finn WF, Gottschalk CW (1975) Autoregulation of blood flow in the rat kidney. Am J Physiol 228:127–133PubMedGoogle Scholar
  2. Balagura S (1966) Uptake and utilization of α-ketoglutarate by rat renal cortical slices. Acta Physiol Lat Am 16:6–12PubMedGoogle Scholar
  3. Balagura S, Pitts RF (1964) Renal handling of α-ketoglutarate by the dog. Am J Physiol 207:483–494PubMedGoogle Scholar
  4. Balagura S, Stone WJ (1967) Renal tubular secretion of alpha-ketoglutarate in dog. Am J Physiol 212:1319–1326PubMedGoogle Scholar
  5. Balagura-Baruch S, Shurland SM, Welbourne TC (1970) Effects of α-ketoglutarate on renal ammonia release in the intact dog. Am J Physiol 218:1070–1075PubMedGoogle Scholar
  6. Bonjour JP, Malvin RL (1969) Renal extraction of PAH, GFR and UNaV in the rat during infusion of angiotensin. Am J Physiol 216:554–558PubMedGoogle Scholar
  7. Boyd TA, Goldstein L (1979) Kidney metabolite levels and ammonia production in acute acid-base alterations in the rat. Am J Physiol 236:E289-E294PubMedGoogle Scholar
  8. Bratton AC, Marshall EK (1939) A new coupling component for sulfanilamide determination. J Biol Chem 128:537–550Google Scholar
  9. Burckhardt G (1984) Sodium-dependent dicarboxylate transport in rat renal basolateral membrane vesicles. Pflügers Arch 401:254–261CrossRefPubMedGoogle Scholar
  10. Chi MMY, Lowry CV, Lowry OH (1978) An improved enzymatic cycle for nicotinamide dinucleotide phosphate. Anal Biochem 89:119–129CrossRefPubMedGoogle Scholar
  11. Churchill PC, Malvin RL (1970) Relation of renal gluconeogenesis to ammonia production in the rat. Am J Physiol 218:353–357PubMedGoogle Scholar
  12. Cohen JJ (1960) High respiratory quotient of dog kidney in vivo. Am J Physiol 199:560–568PubMedGoogle Scholar
  13. Cohen JJ, Wittmann E (1963) Renal utilization and excretion of α-ketoglutarate in dog: effect of alkalosis. Am J Physiol 204: 795–811PubMedGoogle Scholar
  14. Cohen JJ, Chesney RW, Brand PH, Neville HF, Blanchard CF (1969) Alpha-ketoglutarate metabolism and K+ uptake by the dog kidney slices. Am J Physiol 217:161–169PubMedGoogle Scholar
  15. Ferrier B, Martin M, Baverel G (1985) Reabsorption and secretion of α-ketoglutarate along the rat nephron: a micropuncture study. Am J Physiol 248:F404-F412PubMedGoogle Scholar
  16. Fonteles MC, Cohen JJ, Black AJ, Wertheim SJ (1983) Support of kidney function by long-chain fatty acids derived from renal tissue. Am J Physiol 244:F235-F246PubMedGoogle Scholar
  17. Klein KL, Wang MS, Torikai S, Davidson W, Kurokawa K (1981) Substrate oxidation by isolated single nephron segments of the rat. Kidney Int 20:29–35CrossRefPubMedGoogle Scholar
  18. Krebs HA (1939) Microdetermination of α-ketoglutaric acid. Biochem J 32:108–112CrossRefGoogle Scholar
  19. LeHir M, Dubach UC (1982) Activities of enzymes of the tricarboxylic acid cycle in segments of the rat nephron. Pflügers Arch 395:239–243CrossRefGoogle Scholar
  20. Narins RG, Passonneau JV (1974) 2-Oxoglutarate. Fluorimetric determination. In: Bergmeyer HU (ed) Methods of enzymatic analysis. Academic Press, New York, pp 1580–1584Google Scholar
  21. Phillips RA, Dole VP, Hamilton PB, Emerson K, Archibald RM, Van Slyke DD (1945) Effects of acute hemorrhagie and traumatic shock on renal function in dogs. Am J Physiol 145:314–336Google Scholar
  22. Selleck BH, Cohen JJ (1965) Specific localization of α-ketoglutarate uptake to dog kidney and liver in vivo. Am J Physiol 208:24–37PubMedGoogle Scholar
  23. Sheridan E, Rumrich G, Ullrich KJ (1983) Reabsorption of dicarboxylic acids from the proximal convolution of the rat kidney. Pflügers Arch 399:18–28CrossRefPubMedGoogle Scholar
  24. Silbernagl S (1980) Tubular reabsorption ofl-glutamine studied by free-flow micropuncture and microperfusion of rat kidney. Int J Biochem 12:9–16CrossRefPubMedGoogle Scholar
  25. Ullrich KJ, Fachold H, Rumrich G, Klöss S (1984) Secretion and contrainminal uptake of dicarboxylic acids in the proximal convolution of the rat kidney. Pflügers Arch 400:241–249CrossRefPubMedGoogle Scholar
  26. Ullrich KJ, Rumrich G, Fritzsch G, Klöss S (1987) Contraluminal para-aminohippurate (PAH) transport in the proximal tubule of the rat kidney. Pflügers Arch 408:38–45CrossRefPubMedGoogle Scholar
  27. Vishwarkarma P (1963) The proximal renal tubular transport of α-ketoglutaric acid. Can J Biochem Physiol 41:1099–1104CrossRefGoogle Scholar
  28. Vurek GG, Pegram SE (1966) Fluorimetric method for the determination of nanogram quantities of inulin. Anal Biochem 16:409–419CrossRefGoogle Scholar
  29. Welbourne TC, Balagura-Baruchs S (1972) Renal metabolism of glutamine in dogs during infusion of α-ketoglutaric acid. Am J Physiol 22:663–666Google Scholar
  30. Wolf AV (1941) Total renal blood flow at any urine flow or extraction fraction. Am J Physiol 133:496–497Google Scholar

Copyright information

© Springer-Verlag 1989

Authors and Affiliations

  • M. Martin
    • 1
  • B. Ferrier
    • 1
  • G. Baverel
    • 1
  1. 1.Centre National de la Recherche Scientifique (UA 1177); Laboratoire de Physiologie Rénale et Métabolique, Faculté de Médecine Alexis CarrelInstitut National de la Santé et de la Recherche Médicale (U 80)Lyon Cedex 08France

Personalised recommendations