Pflügers Archiv - European Journal of Physiology

, Volume 413, Issue 3, pp 217–224 | Cite as

Transport and utilization of α-ketoglutarate by the rat kidney in vivo

  • M. Martin
  • B. Ferrier
  • G. Baverel
Transport Processes, Metabolism and Bendocrinology; Kidney, Gastrointestinal Tract, and Exocrine Glands


In order to establish the characteristics of net renal transport and utilization of α-ketoglutarate (α-KG) in the rat, we have precisely quantified the renal blood flow, the urinary flow and the rates of α-KG delivery, filtration, reabsorption or secretion, excretion, uptake or production by an in vivo rat kidney preparation. In normal rats, α-KG uptake was higher than α-KG reabsorption at both endogenous and elevated plasma α-KG concentrations; thus, a net peritubular transport, which was the main supplier of α-KG to the renal cells, took place. Saturation of reabsorption and peritubular transport of α-KG occurred at blood α-KG concentrations about 30 and 150 times above normal, respectively. Acute metabolic acidosis was found to have no effect on renal handling of α-KG. At endogenous plasma α-KG concentrations, alkalosis converted net renal uptake into net renal production of α-KG resulting in addition of α-KG by the renal cells both to blood and to the luminal fluid. Elevation of blood α-KG concentration restored the renal uptake of α-KG. This uptake, which was entirely accounted for by the peritubular transport of α-KG, reached a maximum which was lower than that observed in normal and acidotic rats.

Key words

Rat Kidney Uptake Transport α-Ketoglutarate Luminal Basolateral Production 


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Copyright information

© Springer-Verlag 1989

Authors and Affiliations

  • M. Martin
    • 1
  • B. Ferrier
    • 1
  • G. Baverel
    • 1
  1. 1.Centre National de la Recherche Scientifique (UA 1177); Laboratoire de Physiologie Rénale et Métabolique, Faculté de Médecine Alexis CarrelInstitut National de la Santé et de la Recherche Médicale (U 80)Lyon Cedex 08France

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