Pflügers Archiv

, Volume 409, Issue 3, pp 328–332 | Cite as

Effects of the antisecretory factor in pigs

  • Stefan Lange
  • Ivar Lönnroth
  • Erik Skadhauge
Transport Processes, Metabolism and Endocrinology; Kidney, Gastrointestinal Tract, and Exocrine Glands


The effect of the antisecretory factor (ASF) on experimental porcine enterotoxin-induced jejunal secretion was tested. The heat-labile enterotoxin (LT) fromEscherichia coli and cholera toxin (CT) was used for challenge in ligated intestinal loops. Less than 10 units of ASF inhibited the LT-induced secretion, while that due to CT required more than 10 units of ASF. ASF was effective only when administered prior to toxin challenge, and could be given either intravenously or intra-intestinally. Mixing of ASF with specific anti-ASF antibodies prior to injection abolished its antisecretory effect. LT- and CT-induced secreted fluid contained equal concentrations of Na+, K+ and Cl, and the ionic concentration was not affected by ASF. Less than 0.1 units of ASF per pituitary gland was present in 3- and 5-week old pigs, while it increased to 4.5 units in 28-week old animals, and to 12.2 units in pigs older than two years. However, after intra-intestinal vaccination with 2.0 mg CT, the pituitary ASF content in the 5-week old animals increased to 2.0 units within 24h.

Key words

Jejunal secretion Pig Enterotoxin Nerve peptide Passive transfer Ion transport Ligated loops 

Abbreviations used


antisecretory factor


cholera toxin


the heat-labile enterotoxin fromEscherichia coli


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Copyright information

© Springer-Verlag 1987

Authors and Affiliations

  • Stefan Lange
    • 1
  • Ivar Lönnroth
    • 2
  • Erik Skadhauge
    • 3
  1. 1.Department of Clinical BacteriologyUniversity of GöteborgGöteborgSweden
  2. 2.Medical MicrobiologyUniversity of GöteborgGöteborgSweden
  3. 3.Institute of Veterinary Physiology and BiochemistryThe Royal Veterinary and Agricultural UniversityCopenhagenDenmark

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