Pflügers Archiv

, Volume 409, Issue 3, pp 328–332 | Cite as

Effects of the antisecretory factor in pigs

  • Stefan Lange
  • Ivar Lönnroth
  • Erik Skadhauge
Transport Processes, Metabolism and Endocrinology; Kidney, Gastrointestinal Tract, and Exocrine Glands

Abstract

The effect of the antisecretory factor (ASF) on experimental porcine enterotoxin-induced jejunal secretion was tested. The heat-labile enterotoxin (LT) fromEscherichia coli and cholera toxin (CT) was used for challenge in ligated intestinal loops. Less than 10 units of ASF inhibited the LT-induced secretion, while that due to CT required more than 10 units of ASF. ASF was effective only when administered prior to toxin challenge, and could be given either intravenously or intra-intestinally. Mixing of ASF with specific anti-ASF antibodies prior to injection abolished its antisecretory effect. LT- and CT-induced secreted fluid contained equal concentrations of Na+, K+ and Cl, and the ionic concentration was not affected by ASF. Less than 0.1 units of ASF per pituitary gland was present in 3- and 5-week old pigs, while it increased to 4.5 units in 28-week old animals, and to 12.2 units in pigs older than two years. However, after intra-intestinal vaccination with 2.0 mg CT, the pituitary ASF content in the 5-week old animals increased to 2.0 units within 24h.

Key words

Jejunal secretion Pig Enterotoxin Nerve peptide Passive transfer Ion transport Ligated loops 

Abbreviations used

ASF

antisecretory factor

CT

cholera toxin

LT

the heat-labile enterotoxin fromEscherichia coli

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References

  1. 1.
    Argenzio RA (1980) Glucose-stimulated fluid absorption in the pig small intestine during the early stage of swine dysentery. Am J Vet Res 41:2000–2006Google Scholar
  2. 2.
    Carpenter CCJ (1972) Cholera and other enterotoxin-related diarrheal diseases. J Infect Dis 126:551–564Google Scholar
  3. 3.
    Cooke HJ (1986) Neurobiology of the intestinal mucosa. Gastroenterology 90:1057–1081Google Scholar
  4. 4.
    Craig IP (1972) The enterotoxic enteropathies. In: Smith H, Pearce JH (eds) Microbial pathogenicity in man and animals. Cambridge University Press, Cambridge. pp 129–155Google Scholar
  5. 5.
    Field M (1976) Regulation of active ion transport in the small intestine. In: Elliot K, Knight I (eds) Acute diarrhea in childhood. Ciba Foundation, Symposium 42. Elsevier, Amsterdam, pp 109–122Google Scholar
  6. 6.
    Hirst TR, Sanchez I, Kapu IB, Hardy JSJ, Holmgren J (1984) Mechanism of toxin secretion byVibrio cholerae investigated in ofEscherichia coli. Proc Natl Acad Sci USA 81:7752–7756Google Scholar
  7. 7.
    Kimberg DV (1974) Cyclic nucleotides and their role in gastrointestinal secretion. Gastroenterology 67:1023–1064Google Scholar
  8. 8.
    Lange S (1982) A rat model for anin vivo assay of enterotoxic diarrhea. Microbiol Lett 15:239–242Google Scholar
  9. 9.
    Lange S, Lönnroth I (1984) Passive transfer of protection against cholera toxin in the rat intestine. Microbiol Lett 24:165–168Google Scholar
  10. 10.
    Lange S, Lönnroth I (1984) Bile and milk from cholera toxin treated rats contain a hormone-like factor which inhibits diarrhea induced by the toxin. Int Arch Allergy Appl Immunol 79:270–275Google Scholar
  11. 11.
    Lange S, Lönnroth I, Palm A, Hydén H (1985) An inhibitory protein of intestinal fluid secretion reverses neuronal GABA transport. Biochem Biophys Res Commun 130:1032–1036Google Scholar
  12. 12.
    Lönnroth I, Lange S (1984) Purification and characterization of a hormone-like factor which inhibits cholera secretion. FEBS Lett 177:104–108Google Scholar
  13. 13.
    Lönnroth I, Lange S (1985) A hormone-like protein from the pituitary gland inhibits intestinal hypersecretion induced by cholera toxin. Regul Pept (Suppl 4) 216–218Google Scholar
  14. 14.
    Lönnroth I, Lange S (1986) Purification and characterization of the antisecretory factor — a protein in the central nervous system and in the gut which inhibits intestinal hypersecretion induced by cholera toxin. Biochim Biophys Acta 883:138–144Google Scholar
  15. 15.
    Lönnroth I,Hannson H-A, Lange S (1984) Intestinal adaption to cyclic AMP-mediated hypersecretion induced by heat-labile enterotoxin ofVibrio cholerae andEscherichia coli. Acta Pathol Microbiol Immunol Scand (B) 92:53–60Google Scholar
  16. 16.
    Morishita T, Hibi T, Asakuva H, Tsuchiya M (1977) Clinical studies on cholera-variation of blood hormones and amines in a course of cholera. In: Fukumi H, Zinnaka Y (eds) Proc 12th Joint Conf US-Japan cooperative medical science program cholera panel. National Institute of Health, Tokyo, pp 65–71Google Scholar
  17. 17.
    Sack RB (1975) Human diarrheal disease caused by enterotoxigenicEscherichia coli. Annu Rev Microbiol 29:333–354Google Scholar
  18. 18.
    Skadhauge E (1978) Analysis of computer models. In: Gupta BL, Moreton RB, Oschman SL, Wall BJ (eds) Transport of ions and water in animals. Academic Press, London, pp 145–165Google Scholar
  19. 19.
    Smyth CI, Olsson E, Moncalvo C, Söderlind O, Örskov F, Örskov I (1981) K99 antigen-positive enterotoxigenicEscherichia coli from piglets with diarrhea in Sweden. J Clin Microbiol 13:252–257Google Scholar

Copyright information

© Springer-Verlag 1987

Authors and Affiliations

  • Stefan Lange
    • 1
  • Ivar Lönnroth
    • 2
  • Erik Skadhauge
    • 3
  1. 1.Department of Clinical BacteriologyUniversity of GöteborgGöteborgSweden
  2. 2.Medical MicrobiologyUniversity of GöteborgGöteborgSweden
  3. 3.Institute of Veterinary Physiology and BiochemistryThe Royal Veterinary and Agricultural UniversityCopenhagenDenmark

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