Pflügers Archiv

, Volume 406, Issue 5, pp 497–501 | Cite as

BAY K 8644-induced oscillations in rabbit gall-bladder transepithelial potential difference

  • C. Pilebæk Hansen
  • N. -H. Holstein-Rathlou
  • O. Frederiksen
Transport Processes, Metabolism and Endocrinology; Kidney, Gastrointestinal Tract, and Exocrine Glands


The effects of the Ca2+-channel activator BAY K 8644 (a novel dihydropyridine) on transepithelial potential difference (Pd), electrical resistance (Rt), and unidirectional Na+-fluxes were studied in the rabbit gall-bladder.

It was observed that BAY K 8644 at concentrations between 10−7 and 10−5 M induced regular oscillations in the transepithelial Pd, without affecting the mean value of Pd (or Rt). The mean oscillatory frequency was 18 mHz (∼1 cycle per min), and the mean amplitude was 30–35 μV. Oscillations were predominantly elicited from the serosal side. 10−5 M BAY K 8644 reduced net Na+-absorption by 16% by inhibiting the mucosa-to-serosa flux. Nifedipine blocked the Pd-oscillations but did not reverse the Na+-transport inhibition.

The observed effects of BAY K 8644 are consistent with activation of Ca2+-channels and an increase in intracellular Ca2+-concentration.

Key words

Pd-oscillations Sodium transport BAY K 8644 Calcium Gall-bladder 


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.


  1. 1.
    Berridge MJ, Lindley BD, Prince WT (1974) Role of calcium and cyclic AMP in controlling fly salivary gland secretion. In: Thorn NA, Petersen OH (eds) Secretory mechanisms of exocrine glands. Munksgaard, Copenhagen, pp 331–343Google Scholar
  2. 2.
    berridge MJ, Rapp PE (1979) A comparative survey of the function, mechanism and control of cellular oscillators. J Exp Biol 81:217–279Google Scholar
  3. 3.
    Frederiksen O, Leyssac PP (1977) Effects of cytochalasin B and dimethylsulphoxide on isosmotic fluid transport by rabbit gall-bladder in vitro. J Physiol (Lond) 265:103–118Google Scholar
  4. 4.
    Frederiksen O, Leyssac PP (1984) Possible role of calcium in the control of gall-bladder fluid absorption. In: Case RM, Lindgard JM, Young JA (eds) Secretion: mechanisms and control. University Press, Manchester, pp 207–211Google Scholar
  5. 5.
    Frömter E (1972) The route of passive ion movement through the epithelium ofNecturus gall-bladder. J Membr Biol 8: 259–301Google Scholar
  6. 6.
    Henin S, Cremaschi D (1975) Transcellular ion route in rabbit gall-bladder. Electric properties of the epithelial cells. Pflügers Arch 355:125–139Google Scholar
  7. 7.
    Hess P, Lansman JB, Tsien RW (1984) Different modes of Ca channel gating behaviour favoured by dihydropyridine Ca agonist and antagonist. Nature 311:538–544Google Scholar
  8. 8.
    Ishii K, Taira N, Yanagisawa T (1985) Differential antagonism by BAY K 8644, a dihydropyridine calcium agonist, of the negative inotropic effects of nifedipine, verapamil, diltiazem and manganese ions in canine ventricular muscle. Br J Pharmacol 84:577–584Google Scholar
  9. 9.
    Kennedy RH, Seifen E (1985) Stimulation frequency alters the inotropic response of atrial muscle to BAY K 8644. Eur J Pharmacol 107:209–214Google Scholar
  10. 10.
    Kinne-Saffran E, Kinne RT (1974) Localization of a calcium-stimulated ATPase in the basal-lateral plasma membranes of the proximal tubule of rat kidney cortex. J Membr Biol 17:263–274Google Scholar
  11. 11.
    Lassalles J-P, Hartmann A, Thellier M (1980) Oscillations of the electric potential of frog skin under the effect of Li+: experimental approach. J Membr Biol 56:107–119Google Scholar
  12. 12.
    Petersen OH, Maruyama Y (1984) Calcium-activated potassium channels and their role in secretion. Nature 307:693–696Google Scholar
  13. 13.
    Rapp PE (1979) An atlas of cellular oscillators. J Exp Biol 81:281–306Google Scholar
  14. 14.
    Rasmussen H, Barrett PQ (1984) Calcium messenger system: an integrated view. Physiol Rev 64:938–984Google Scholar
  15. 15.
    Scharff O, Foder B (1982) Rate constants for calmodulin binding to Ca2+-ATPase in erythrocytes. Biochim Biophys Acta 691:133–143Google Scholar
  16. 16.
    Scharff O, Foder B, Skibsted U (1983) Hysteretic activation of the Ca2+ pump revealed by calcium transients in human red cells. Biochim Biophys Acta 730:295–305Google Scholar
  17. 17.
    Schramm M, Thomas G, Towart R, Franckowiak G (1983) Novel dihydropyridine with positive inotropic action through activation of Ca2+-channels. Nature 303:535–537Google Scholar
  18. 18.
    Thomas G, Chung M, Cohen CJ (1985) A dihydropyridine (BAY K 8644) that enhances calcium currents in guinea pig and calf myocardial cells. Circ Res 56:87–96Google Scholar
  19. 19.
    Van Os CH, Ghijsen WEJM, De Jonge H (1981) High affinity Ca-ATPase in basolateral plasma membrane of rat duodenum and kidney cortex. In: Bronner F, Peterlik M (eds). Calcium and phosphate transport across biomembranes. Academic Press, New York, p 159Google Scholar
  20. 20.
    Vestergaard-Bogind B, Bennekou P (1982) Calcium-induced oscillations in K+ conductance and membrane potential of human erythrocytes mediated by the ionophore A23187. Biochim Biophys Acta 688:37–44Google Scholar
  21. 21.
    Windhager EE, Taylor A (1983) Regulatory role of intracellular calcium ions in epithelial Na transport. Annu Rev Physiol 45:519–532Google Scholar
  22. 22.
    Winfree AT (1967) Biological rhythms and the behavior of populations of coupled oscillators. J Theoret Biol 16:15–42Google Scholar

Copyright information

© Springer-Verlag 1986

Authors and Affiliations

  • C. Pilebæk Hansen
    • 1
  • N. -H. Holstein-Rathlou
    • 1
  • O. Frederiksen
    • 1
  1. 1.The University Institute of Experimental MedicineCopenhagenDenmark

Personalised recommendations