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Pflügers Archiv

, Volume 403, Issue 1, pp 50–54 | Cite as

36Cl fluxes in dispersed rat submandibular acini: Effects of acetylcholine and transport inhibitors

  • J. Ricardo Martinez
  • Nancy Cassity
Transport Processes, Metabolism and Endocrinology; Kidney, Gastrointestinal Tract, and Exocrine Glands

Abstract

Acini isolated from the rat submandibular gland showed uptake of36Cl in the absence of added test substances. A steady state of36Cl content corresponding to 9.5±0.7 nM/mg protein was attained in 4–5 min and maintained for 25–30 min. This uptake of36Cl was reduced 40–50% by previous exposure to 10−3 M furosemide, 10−3 M ouabain, or K+-free medium. The steady state of36Cl content was reduced 34% by 10−6 M acetylcholine while exposure of the acini to the stilbene sulfonic DIDS (10−3 M) did not modify36Cl uptake either in the absence (basal) or presence of acetylcholine. Acetylcholine caused a rapid reduction of36Cl from tracer-preloaded acini (42% in 1 min), while furosemide induced a slower reduction of tracer content (45% in 9 min). Acetylcholine increased the rate of reduction of tracer content induced by furosemide and the latter caused a further reduction in36Cl content of acini previously exposed to acetylcholine. It is concluded that a furosemide-sensitive Cl uptake is present in rat submandibular acini, which depends on the Na+ gradient generated by the Na, K pump and on external K+. These findings suggest that a Na−K−Cl cotransport system driven by the Na, K pump is present in salivary acinar cells. Acetylcholine induces a rapid net efflux of36Cl, which probably masks any effect that this secretagogue may have on Cl uptake. The lack of effect of DIDS on36Cl uptake suggests that anion exchange transport systems are either not present in rat submandibular acini or can only be ascertained under different incubation conditions.

Key words

Submandibular acini 36Cl fluxes Acetyl-choline Transport inhibitors 

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Copyright information

© Springer-Verlag 1985

Authors and Affiliations

  • J. Ricardo Martinez
    • 1
  • Nancy Cassity
    • 1
  1. 1.Departments of Child Health and PhysiologyUniversity of Missouri School of MedicineColumbiaUSA

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