Pflügers Archiv

, Volume 403, Issue 1, pp 28–34 | Cite as

Stimulation by glucagon and PTH of Ca and Mg reabsorption in the superficial distal tubule of the rat kidney

  • C. Bailly
  • N. Roinel
  • C. Amiel
Transport Processes, Metabolism and Endocrinology; Kidney, Gastrointestinal Tract, and Exocrine Glands


The effects of glucagon and PTH on electrolyte reabsorption in the distal tubule were investigated in rats deprived of vasopressin, calcitonin, PTH, and glucagon. Micropunctures of distal tubule, at a late and an early site of a same nephron, have been performed in 23 rats, nine control, seven infused with glucagon (5 ng·min−1·100 g−1 b.w.) and seven with PTH (5 mU·min−1·100 g−1 b.w.). The Ca and Mg reabsorptive capacity of the distal segment was increased by glucagon and by PTH. Moreover, fractional Na and Cl reabsorption was significantly higher than in control during PTH administration. A K secretion appeared during the administration of both hormones. No phosphate net transport was observed in any group. Finally, the data presented here, together with those previously reported, indicate that the increase of Ca and Mg renal reabsorption observed with glucagon and PTH results from an effect located in both Henle's loop, where the bulk of Ca and Mg is reabsorbed, and the distal tubule.


Phosphate Human Physiology Glucagon Calcitonin Vasopressin 
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  1. 1.
    Amiel C, Kuntziger H, Richet G (1970) Micropuncture study of handling of phosphate by proximal and distal nephron in normal and parathyroidectomized rat. Evidence for distal reabsorption Pflügers Arch 317:93–100Google Scholar
  2. 2.
    Bailly C, Amiel C (1982) Effect of glucagon on magnesium renal reabsorption in the rat. Pflügers Arch 392:360–365Google Scholar
  3. 3.
    Bailly C, Imbert-Teboul M, Chabardès D, Hus-Citharel A, Montégut M, Clique A, Morel F (1980) The distal nephron of rat kidney: a target site for glucagon. Proc Natl Acad Sci USA 77:3422–3424Google Scholar
  4. 4.
    Bailly C, Roinel N, Amiel C (1984) PTH-like glucagon stimulation of Ca and Mg reabsorption in Henle's loop of the rat. Am J Physiol 246:F205-F212Google Scholar
  5. 5.
    Chabardès D, Imbert M, Clique A, Montégut M, Morel F (1975) PTH sensitive adenyl cyclase activity in different segments of the rabbit nephron. Pflügers Arch 354:229–239Google Scholar
  6. 6.
    Chen PS, Jr, Toribara TY, Warner H (1956) Microdetermination of phosphorus. Anal Chem 28:1756–1758Google Scholar
  7. 7.
    Costanzo LS, Windhager EE (1978) Calcium and sodium transport by the distal convoluted tubule of the rat. Am J Physiol 235:F492-F506Google Scholar
  8. 8.
    Costanzo LS, Windhager EE (1980) Effects of PTH, ADH, and cyclic AMP on distal tubular Ca and Na reabsorption. Am J Physiol 239:F478-F485Google Scholar
  9. 9.
    Efendic S, Lins PE, Luft R (1978) Somatostatin and insulin secretion. Metabolism 27:1275–1281Google Scholar
  10. 10.
    Elalouf JM, Roinel N, de Rouffignac C (1983) Stimulation by human calcitonin of electrolyte transport in distal tubules of rat kidney Pflügers Arch 399:111–118Google Scholar
  11. 11.
    Elalouf JM, Roinel N, de Rouffignac C (1984) Effects of antidiuretic hormone on electrolyte reabsorption and secretion in distal tubules of rat kidney. Pflügers Arch (in press)Google Scholar
  12. 12.
    Emmanouel DS, Jaspan JB, Rubenstein AH, Huen AHJ, Fink E, Katz AI (1978) Glucagon metabolism in the rat. J Clin Invest 62:6–13Google Scholar
  13. 13.
    Harris CA, Burnatowska MA, Seely JF, Sutton MAL, Quamme GA, Dirks JH (1979) Effects of parathyroid hormone on electrolyte transport in the hamster nephron. Am J Physiol 236:F342-F348Google Scholar
  14. 14.
    Imai M (1981) Effects of parathyroid hormone and N6, O2 dibutyryl cyclic AMP on Ca2+ transport across the rabbit distal nephron segments perfused in vitro. Pflügers Arch 390:145–151Google Scholar
  15. 15.
    Kaissling B, Kriz W (1982) Axial heterogeneity of the “distal tubule”. Contr Nephrol 33:29–47Google Scholar
  16. 16.
    Morel F, Roinel N (1969) Application de la microsonde électronique à l'analyse élénmentaire quantitative d'échantillons liquides d'un volume inférieur à 10−9 l. J Chim Phys-Chim Biol 66:1084–1091Google Scholar
  17. 17.
    Morel F, Chabardès D, Imbert-Teboul M, Le Bouffant F, Hus-Citharel A, Montégut M (1982) Multiple hormonal control of adenylate cyclase in distal segments of the rat kidney. Kidney Int 21:555–562Google Scholar
  18. 18.
    Quamme GA (1982) Effect of hypercalcemia on renal tubular handling of calcium and magnesium. Can J Physiol Pharmacol 60:1275–1280Google Scholar
  19. 19.
    Quamme GA, Dirks JH (1980) Intraluminal and contraluminal magnesium on magnesium and calcium transfer in the rat nephron. Am J Physiol 238:F187-F198Google Scholar
  20. 20.
    Shareghi GR, Stoner LC (1978) Calcium transport across segments of the rabbit distal nephron in vitro. Am J Physiol 235:F367-F375Google Scholar
  21. 21.
    Snedecor GW, Cochran WG (1972) Statistical methods, 6th edn. The Iowa State University Press, Ames, Iowa, USAGoogle Scholar
  22. 22.
    Wright FS (1977) Site and mechanisms of potassium transport along the renal tubule. Kidney Int 11:415–432Google Scholar

Copyright information

© Springer-Verlag 1985

Authors and Affiliations

  • C. Bailly
    • 1
  • N. Roinel
    • 2
  • C. Amiel
    • 1
  1. 1.Unité INSERM 251, Département de Physiologie, Faculté Xavier BichatUniversité Paris 7ParisFrance
  2. 2.Laboratoire de Physiologie Physico-Chimique, Département de BiologieCentre d'Etudes Nucléaires de SaclayGif-sur-Yvette, CedexFrance

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