Pflügers Archiv

, Volume 403, Issue 1, pp 7–12 | Cite as

Fixed acid and carbon dioxide bohr effects as functions of hemoglobin-oxygen saturation and erythrocyte pH in the blood of the frog,Rana temporaria

  • Rufus M. G. Wells
  • Roy E. Weber
Heart, Circulation, Respiration and Blood; Environmental and Exercise Physiology


Using a thin film, dynamic recording technique, the pH sensitivity of the oxygen equilibrium (Bohr effect) of whole blood in the frogRana temporaria, and its dependence on CO2 and fixed acids and on plasma and erythrocyte pH values were measured. Under standard conditions (20°C,PCO2=14.7 mm Hg, pH=7.65) the oxygen equilibrium could be described by a P50 value of 38 mm Hg andn50 of 1.8. Hill plots of the oxygen equilibria showed increased cooperativity in oxygen binding with increasing saturation (n20 ≃ 1.2,n80 ≃ 4.0). Values of the fixed acid and CO2 Bohr factors ({ie7-1} and {ie7-2}, respectively) were similar at specific saturations (S20, 50, 80) but showed saturation dependence with high values occurring at high saturation. The same statements also hold for the intracellular Bohr factors (derived from the relation between blood P50 and erythrocyte pH) although the values of both {ie7-3} and {ie7-4} now were greater than those related to blood pH.

Key words

Blood and erythrocytic Bohr effects Frog CO2 effect Hill coefficient O2 affinity ATP 


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Copyright information

© Springer-Verlag 1985

Authors and Affiliations

  • Rufus M. G. Wells
    • 1
  • Roy E. Weber
    • 1
  1. 1.Institute of BiologyOdense UniversityOdense MDenmark

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