Pflügers Archiv

, Volume 394, Issue 2, pp 144–149 | Cite as

Effect of procaine on potassium permeability of canine tracheal smooth muscle

  • Yuji Imaizumi
  • Minoru Watanabe
Excitable Tissues and Central Nervous Physiology

Abstract

Procaine depolarized the cell membrane, and initiated oscillations and spike-like potentials in canine tracheal smooth muscle, at concentrations between 1 and 5 mM, while higher concentrations of this drug suppressed the spontaneous activities. Inhibitory effects of procaine on86Rb-efflux were not evident in normal solution. In high K solution, procaine decreased the rate of86Rb-efflux, in a dose dependent manner, and a Scatchard plot suggested two sites of action for procaine. The site with a higher affinity (KD=0.26 mM) may directly regulate the K permeability and interact with procaine in a one to one manner. In high concentrations (≧10 mM), procaine interacted at another site and the tracheal smooth muscle which contracted in high K solution relaxed almost completely. Thus, the decrease in86Rb-efflux by high concentrations of procaine may partly result from a decrease in intracellular Ca concentration. It is proposed that procaine has the dual effect by inhibiting the K conductance: (1) depolarization of cell membrane, (2) increase in membrane excitability. These actions would explain the spontaneous electrical activity induced by procaine in canine tracheal smooth muscle.

Key words

Tracheal smooth muscle Potassium permeability 86Rb-efflux Procaine 

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Copyright information

© Springer-Verlag 1982

Authors and Affiliations

  • Yuji Imaizumi
    • 1
  • Minoru Watanabe
    • 1
  1. 1.Department of Chemical Pharmacology, Faculty of Pharmaceutical SciencesNagoya City UniversityNagoyaJapan

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