Molecular and General Genetics MGG

, Volume 147, Issue 3, pp 331–335 | Cite as

ColE plasmid replication in DNA polymerase I-deficient strains ofEscherichia coli

  • William Tacon
  • David Sherratt
Article

Summary

Replication of the non-conjugative plasmids ColE1, ColE2 and ColE3 has been examined in a number of DNA polymerase I-deficient strains, two of which contain the amber mutationpolA1 along with either of two temperature-sensitivesupF amber suppressors. These latter two strains produce reduced amounts of DNA polymerase I polymerizing activity of similar, if not identical properties to that produced bypolA+ strains. Our results indicate that the ColE plasmids require different amounts of DNA polymerase I for stable plasmid maintenance. Moreover whereas all three plasmids are maintained in a strain defective in the 5′→3′ exonuclease activity of DNA polymerase I, ColE2 and ColE3 are not stably maintained between 30° and 43° in a number of DNA polymerase I-deficient strains that are temperature-sensitive for ColE1 replication.

Keywords

Identical Property Polymerize Activity Exonuclease Activity Stable Plasmid Plasmid Replication 

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Copyright information

© Springer-Verlag 1976

Authors and Affiliations

  • William Tacon
    • 1
  • David Sherratt
    • 1
  1. 1.School of Biological SciencesUniversity of SussexBrightonEngland

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