ColE plasmid replication in DNA polymerase I-deficient strains ofEscherichia coli
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Replication of the non-conjugative plasmids ColE1, ColE2 and ColE3 has been examined in a number of DNA polymerase I-deficient strains, two of which contain the amber mutationpolA1 along with either of two temperature-sensitivesupF amber suppressors. These latter two strains produce reduced amounts of DNA polymerase I polymerizing activity of similar, if not identical properties to that produced bypolA+ strains. Our results indicate that the ColE plasmids require different amounts of DNA polymerase I for stable plasmid maintenance. Moreover whereas all three plasmids are maintained in a strain defective in the 5′→3′ exonuclease activity of DNA polymerase I, ColE2 and ColE3 are not stably maintained between 30° and 43° in a number of DNA polymerase I-deficient strains that are temperature-sensitive for ColE1 replication.
KeywordsIdentical Property Polymerize Activity Exonuclease Activity Stable Plasmid Plasmid Replication
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