ColE plasmid replication in DNA polymerase I-deficient strains ofEscherichia coli
- 81 Downloads
Replication of the non-conjugative plasmids ColE1, ColE2 and ColE3 has been examined in a number of DNA polymerase I-deficient strains, two of which contain the amber mutationpolA1 along with either of two temperature-sensitivesupF amber suppressors. These latter two strains produce reduced amounts of DNA polymerase I polymerizing activity of similar, if not identical properties to that produced bypolA+ strains. Our results indicate that the ColE plasmids require different amounts of DNA polymerase I for stable plasmid maintenance. Moreover whereas all three plasmids are maintained in a strain defective in the 5′→3′ exonuclease activity of DNA polymerase I, ColE2 and ColE3 are not stably maintained between 30° and 43° in a number of DNA polymerase I-deficient strains that are temperature-sensitive for ColE1 replication.
KeywordsIdentical Property Polymerize Activity Exonuclease Activity Stable Plasmid Plasmid Replication
Unable to display preview. Download preview PDF.
- Clewell, D.B., Helinski, D.R.: Effect of growth conditions on the formation of supercoiled, ColE1 deoxyribonucleic acid and protein inEscherichia coli. J. Bact.110, 1135–1146 (1972)Google Scholar
- Durkacz, B.W., Kennedy, C.K., Sherratt, D.J.: Plasmid replication and the induced synthesis of colicins E1 and E2 inEscherichia coli. J. Bact.117, 940–946 (1974)Google Scholar
- Durkacz, B.W., Sherratt, D.J.: Segregation kinetics of colicinogenic factor ColE1 from a bacterial population temperature sensitive for DNA polymerase I. Molec. gen. Genet.121, 71–75 (1973)Google Scholar
- Glickman, B.W., van Sluis, C.A., Heigneker, H.L., Rorsch, A.: A mutant ofEscherichia coli K12 deficient in the 5′→3′ exonucleolytic activity of DNA polymerase I. I. General characterization. Molec. gen. Genet.124, 69–82 (1973)Google Scholar
- Goebel, W.: Replication of the DNA of ColE1 at the restrictive temperature in a DNA replication mutant thermosensitive for DNA Polymerase III. Nature (Lond.) New Biol.237, 67–71 (1972)Google Scholar
- Grindley, N.D.F., Kelley, W.S.: Effects of different alleles of theE. coli K12polA gene on the replication of non-transferring plasmids. Molec. gen. Genet.143, 311–318 (1976)Google Scholar
- Kennedy, C.K.: Induction of colicin production by high temperature or inhibition of protein synthesis. J. Bact.108, 10–19 (1971)Google Scholar
- Kennedy, C.K.: Induced biosynthesis of colicins and the properties of a temperature-sensitive mutant colicin. Ph.D. Thesis, M.I.T. (1972)Google Scholar
- Kingsbury, D.T., Helinski, D.R.: Temperature-sensitive mutants for the replication of plasmids inEscherichia coli: Requirement for DNA polymerase I in the replication of the plasmid ColE1. J. Bact.114, 1116–1124 (1973)Google Scholar
- Lucia, P.de, Cairns, J.: Isolation of anE. coli strain with a mutation affecting DNA polymerase. Nature (Lond.)224, 1164–1166 (1969)Google Scholar
- Smith, J.D., Barnet, L., Brenner, S., Russell, R.L.: More mutant tyrosine transfer ribonucleic acids. J. molec. Biol.54, 1–14 (1970)Google Scholar