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Altered formation of dye-induced lysosomes in mouse fibroblasts due to growth in “deficient” media

Light and electron microscopic observations

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Summary

Mouse fibroblasts were grown in a ‘deficient’ medium consisting solely of Hank's salt solution. Treatment of cells after 48 hours in deficient medium with the cationic dye, mepacrine, resulted in an altered formation of lysosomes. Cells grown in full medium form dense, multi-vesiculated lysosomes in response to mepacrine. Cells grown in deficient medium form large electron lucent vacuoles containing peripherally located dense globules. The significance of the observations in relation to the deficiency of the medium is discussed.

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Authors and Affiliations

  1. Anatomisches Institut der Universität Freiburg, Lehrstuhl II, Freiburg i. Br., West Germany

    R. L. Snipes, D. Wittekind & V. Kretschmer

  2. Zentrum für Anatomie und Zytobiologie, am Klinikum der Justus Liebig-Universität, Friedrichstraße 24, 6300, Giessen, Germany

    R. L. Snipes, D. Wittekind & V. Kretschmer

Authors
  1. R. L. Snipes
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  2. D. Wittekind
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  3. V. Kretschmer
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Additional information

A portion of this work was accomplished by R. L. Snipes at the III Department of Pathology, University of Helsinki, Helsinki, Finland. Special thanks are extended to Prof. L. Saxén and Dr. J. Wartiovaara of this department for the generous use of the EM unit.

We are indebted to Dr. D. Zissler, Institut für Biologie I der Universität Freiburg for the use of the Zeiss electron microscope.

Recipient of an Alexander von Humboldt Fellowship. R. L. Snipes is particularly grateful to the Alexander von Humboldt Stiftung for granting the fellowship.

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Snipes, R.L., Wittekind, D. & Kretschmer, V. Altered formation of dye-induced lysosomes in mouse fibroblasts due to growth in “deficient” media. Z.Zellforsch 147, 219–235 (1974). https://doi.org/10.1007/BF00582797

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  • DOI: https://doi.org/10.1007/BF00582797

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