Abstract
The “deformability” of rat erythrocytes can be gradually decreased by an in vitro treatment with the SH-oxidizing agent diamide. Despite of this reduced deformability the cells are retained in the circulation for many hours when reinfused into the rat. Cells rigidified with glutaraldehyde are even less deformable than diamide treated cells, but also survive for many hours.
In contrast to rigidified cells of the normal volumes swollen rigidified cells obtained by a heat treatment of erythrocytes are rapidly eliminated. The results support the notion that the “recognition” preceding the elimination of senescent and damaged cells of normal volumes by the reticulo-endothelial system is not solely based on their diminished deformability.
Similar content being viewed by others
References
Baxter A, Beeley JG (1978) Surface carbohydrates of aged erythrocytes. Biochem Biophys Res Commun 83:466–471
Beutler E, Duron O, Mikus Kelly B (1963) Improved method for determination of blood glutathione. J Lab Clin Med 61:882–888
Chen LT, Weiss L (1973) The role of the sinus wall in the passage of erythrocytes through the spleen. Blood 41:529–537
De Boisfleury A, Mohandas N (1977) Antibody-induced spherocytic Anemia II. Splenic passage and sequestration of red cells. Blood Cells 3:197–208
Driessen G, Haest CWM, Heidtmann H, Kamp D, Schmid-Schönbein H (1980) Effect of reduced red cell deformability on their flow velocity in capillaries of rat mesentery. Pflügers Arch 388:75–78
Fischer TM, Haest CWM, Stöhr M, Kamp D, Deuticke B (1978) Selective alteration of erythrocyte deformability by SH-reagents. Evidence for an involvement of Spectrin in membrane shear elasticity. Biochim Biophys Acta 510:270–282
Haest CWM, Fischer TM, Plasa G, Deuticke B (1980) Stabilization of erythrocyte shape by a chemical increase of membrane shear stiffness. Blood Cells 6:539–553
Heusinkveld RS, Goldstein DA, Weed RI, La Celle P (1977) Effect of protein modification on erythrocyte membrane mechanical properties. Blood Cells 3:175–182
Jacob HS, Jandl JH (1962) Effects of sulfhydryl inhibition on red blood cells. II. Studies in vivo. J Clin Invest 41:1514–1523
Kay MMB (1975) Mechanism of removal of senescent cells by human macrophages in situ. Proc Natl Acad Sci 72:3521–3525
Kolb H, Schudt C, Kolb-Bachofen V, Kolb HA (1978) Cellular recognition by rat liver cells of neuraminidase-treated erythrocytes. Exp Cell Res 113:319–325
Murakarni T, Fujita T, Miyoslin M (1973) Closed circulation in the rat spleen as evidenced by scanning electron microscopy of vascular casts Experientia 29:1374–1375
Oster ZH, Som P, Atkins HL, Cicale NR (1978) Factors affecting splenic deposition of labeled red blood cells in the rat. J Nucl Med Biol 5:223–228
Owen CA, Orvis AL, Kiely JM (1966) Sequestration of erythrocytes by rat spleen. Am J Physiol 211:273–280
Snook T (1964) Studies on the perifollicular region of the rat's spleen. Anat Rec 148:149–160
Vaupel P, Ruppert H, Hutten H (1977) Splenic blood flow and intrasplenic flow distribution in rats. Pflügers Arch 369:193–201
Weed RI, Reed CF (1966) Alterations leading to red cell destruction. Am J Med 41:681–690
Author information
Authors and Affiliations
Additional information
For explanation of quotation marks see note added in proof
This study was supported by the Deutsche Forschungsgemeinschaft (SFB 109 and 160)
Rights and permissions
About this article
Cite this article
Haest, C.W.M., Driessen, G.K., Kamp, D. et al. Is “deformability” a parameter for the rate of elimination of erythrocytes from the circulation?. Pflugers Arch. 388, 69–73 (1980). https://doi.org/10.1007/BF00582630
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF00582630