Antonie van Leeuwenhoek

, Volume 62, Issue 3, pp 167–171 | Cite as

Isolation of auxotrophic mutants of the methylotrophic yeastCandida boidinii and determination of its ploidy

  • Ivana Janatová
Article
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Abstract

Auxotrophic mutations in the methylotrophic yeast strainCandida boidinii 11Bh were induced by different mutagens and their combinations (nitrosoguanidine, UV light, HNO2+UV). Majority of the mutants obtained carried defects in histidine, arginine, proline and/or adenine biosynthetic pathways. His- mutants were distributed into four complementation groups using the protoplasts fusion technique. Ploidy determination ofCandida boidinii 11Bh was performed by measuring its DNA content and by following its survival after chemical mutagens treatment. The DNA content of this strain was found to be similar to that of aSaccharomyces cerevisiae diploid strain. Also the kinetics of survival ofCandida boidinii cells indicate thatCandida boidinii 11Bh is a diploid.

Key words

Candida boidinii mutagenesis auxotrophic mutant ploidy 

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Copyright information

© Kluwer Academic Publishers 1992

Authors and Affiliations

  • Ivana Janatová
    • 1
  1. 1.Institute of MicrobiologyCzechoslovak Academy of SciencesPrague 4Czechoslovakia

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