Abstract
We investigated the influence of inositol triphosphate (IP3), trifluoperazine (TFP), and perhexiline on the calcium sensitivity of freeze-dried frog semitendinosus muscle fibres. Further, the effect of IP3 on calcium release from the sarcoplasmic reticulum (SR) of frog semitendinosus fibres skinned by saponin was studied. IP3 decreased the calcium sensitivity of freeze-dried frog skeletal muscle fibres and failed to induce a calcium release from SR of saponin-skinned fibres. Freeze-dried frog skeletal muscle fibres were strongly sensitized for calcium by TFP and perhexiline.
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Arner A, Pfitzer G, Rüegg JC (1985) Trifluoperazine and fendiline influence calcium sensitivity of skinned cardiac and smooth muscle fibres differentially. Pflügers Arch 405 (Suppl 2):R14 (abstract)
Ashley CC, Griffith PJ, Lea TJ, Tregear RT (1985) Does inositol trisphosphate induce tension development in skinned muscle fibres of barnacle and frog? J Physiol 3698:185P
Cheung WY (1980) Calmodulin plays a pivotal role in cellular regulation. Science 207:19–27
Cohen SM, Burt CT (1977)31P nuclear magnetic relaxation studies of phosphocreatine in intact muscle: determination of intracellular free magnesium. Proc Natl Acad Sci USA 74:4271–4275
Donaldson SK, Goldberg ND, Walseth TF, Huetteman DA (1987) Inositol trisphosphate stimulates calcium release from peeled skeletal muscle fibres. Biochim Biphys Acta 927:92–99
Drabikowski W, Dalbarno DC, Levine BA, Gergely J, Grabarek Z, Leavis PC (1985) Solution conformation of the C-terminal domain of skeletal troponin C. Cation, trifluoperazine, and troponin I binding effects. Eur J Biochem 151:17–28
Fabiato A, Fabiato F (1979) Calculator program for computing the composition of the solutions containing multiple metals and ligands used for experiments in skinned muscle cells. J Physiol (Paris) 75:463–505
Lea TJ, Griffiths PJ, Tregear RT, Ashley CC (1986) An examination of the ability of inositol 1,4,5-trisphosphate to induce calcium release and tension development in skinned skeletal muscle fibres of frog and crustacea. FEBS Lett 207:153–161
Mikos GJ, Snow TR (1987) Failure of inositol 1,4,5-trisphosphate to elicit or potentiate Ca2+ release from isolated skeletal muscle sarcoplasmic reticulum. Biochim Biophys Acta 927:256–260
Morano I, Piazzesi G, Rüegg JC (1988) Myofibrillar calcium sensitivity modulation: influence of light chain phosphorylation and positive inotropic drugs on skinned frog skeletal muscle. In: Sugi H, Pollack GH (eds) Molecular mechanism of muscle contraction. Plenum Press, New York (in press)
Nosek TM, Williams MF, Zeigler ST, Godt RE (1986) Inositol trisphosphate enhances calcium release in skinned cardiac and skeletal muscle. Am J Physiol 250:C807-C811
Persechini A, Stull JT, Cooke R (1985) The effect of myosin phosphorylation on the contractile properties of skinned rabbit skeletal muscle. J Biol Chem 260:7951–7954
Piazzesi G, Morano I, Rüegg JC (1987) Effect of sulmazole and pimobendan on contractility of skinned fibres from frog skeletal muscle. Arzneimittelforschung 37:1141–1143
Rüegg JC (1986) Calcium in muscle activation. A comparative approach. Springer, Berlin Heidelberg New York
Scherer NM, Ferguson JE (1985) Inositol 1,4,5-trisphosphate is not effective in releasing calcium from skeletal sarcoplasmic reticulum microsomes. Biochem Biophys Res Commun 128: 1064–1070
Solaro RJ, Rüegg JC (1982) Stimulation of Ca2+ binding and ATPase activity of dog cardiac myofibrils by AR-L 115 BS, a novel cardiotonic agent. Circ Res 51:290–294
Somlyo AP (1985) The messenger across the gap. Nature 316:298–299
Stienen GJM, Güth K, Rüegg JC (1983) Force and force transients in skeletal muscle fibres of the frog skinned by freeze-drying. Pflügers Arch 397:272–276
Thieleczek R, Heilmeyer LMG Jr (1986) Inositol 1,4,5-trisphosphate enhances Ca2+-sensitivity of the contractile mechanism of chemically skinned rabbit skeletal muscle fibres. Biochem Biophys Res Commun 135:662–669
Tkachuk VA, Baldenkov GN, Feoktistov IA, Men'shikov MY, Quast U, Herzig JW (1987) Metofenazate as a more selective calmodulin inhibitor than trifluoperazine. Arzneimittelforschung 37:1013–1017
Vergara J, Tsien RY (1985) Inositol trisphosphate induced contractures in frog skeletal muscle fibres. Biophys J 47:531 a (abstract)
Vergara J, Tsien R, Delay M (1985) Inositol 1,4,5-trisphosphate: A possible chemical link in excitation contraction coupling in muscle. Proc Natl Acad Sci USA 82:6352–6356
Volpe P, Salviati G, De Virgilio F, Pozzan T (1985) Inositol 1,4,5-trisphosphate induces calcium release from sarcoplasmic reticulum of skeletal muscle. Nature 316:347–349
Walker JW, Somlyo AV, Goldman YE, Somlyo AP, Trentham DR (1987) Kinetics of smooth and skeletal muscle activation by laser pulse photolysis of caged inositol 1,4,5-trisphosphate. Nature 327:249–252
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Isac, M., Morano, I. & Rüegg, J.C. Alteration of calcium sensitivity of skinned frog skeletal muscle fibres by inositol triphosphate and calmodulin antagonists. Pflugers Arch. 412, 253–257 (1988). https://doi.org/10.1007/BF00582505
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DOI: https://doi.org/10.1007/BF00582505