Summary
The ultrastructure of epidermis pretreated with 0,1 N sodium hydroxide, etherethanol mixture and 0,5 M sodium thioglycolate was studied.
Sodium hydroxide dissolves the keratohyalin granules and the interfibrillar material in the keratin. It has no effect on the epidermal and keratin fibers and on the marginal dense band of the horny cells. It “produces” a keratin pattern in the psoriatic horny layer.
After sodium thioglycolate treatment the keratohyalin disappears, the epidermal and keratin fibers show a periodicity and the cementing material in the horny cells is more opaque.
After lipid extraction the structure of keratohyalin becomes inhomogenous. The effect is somewhat similar to that of sodium thioglycolate but there is no periodicity in the horny layer.
The intercellular contact layers of desmosomes as well as the Selby-Odland bodies disappear after each kind of treatment.
The different effect of sodium thioglycolate on the horny layer and on the keratohyalin implies that the material of the keratohyalin and of the cementing substance are not identical.
Zusammenfassung
Die Wirkung von 0,1 N NaOH, Äther-Alkohol-Extraktion und 0,5 M Na-Thioglykolat-Behandlung auf die Ultrastruktur des Epithels wurde untersucht.
NaOH löst Keratohyalin und die Bindesubstanz von Keratin; die Epithel- und Keratinfasern hingegen bleiben unberührt, ebenso die Hornzellmembranen. Bei Psoriasis bewirkt es das Erscheinen des „Keratinmusters“.
Nach Na-Thioglykolat-Behandlung verschwindet das Keratohyalin, im Gebiet der Epithel- und Keratinfasern erscheint Periodizität, der Kontrast der Keratin-Bindesubstanz wird stärker.
Die Extraktion der Lipide bewirkt im Keratohyalin Flecken. Die Wirkung ist ähnlich derjenigen des Na-thioglykolat, aber ohne daß Periodizität in Hornschicht auftritt.
Sowohl die intercelluläre Kontaktschicht der Desmosomen, als auch die Selby-Odlandsche Körper verschwinden nach sämtlichen Vorbehandlungen.
Die verschiedene Wirkung der Na-Thioglykolat-Behandlung auf Hornschicht und Keratohyalin spricht dagegen, daß Keratohyalin und Matrixsubstanz identisch sind.
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Nagy-Vezekényi, K., Zs.-Nagy, I. & Török, E. Die wirkung einiger lösungsmittel auf die epidermis. Arch. Derm. Res. 252, 53–61 (1975). https://doi.org/10.1007/BF00582431
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DOI: https://doi.org/10.1007/BF00582431