Applied Microbiology and Biotechnology

, Volume 22, Issue 5, pp 301–305 | Cite as

Biodegradation of 4-chlorophenol by entrappedAlcaligenes sp. A 7-2

  • F. Westmeier
  • H. J. Rehm


The degradation of 4-chlorophenol by free and by Ca-alginate-immobilized cells ofAlcaligenes sp. A 7-2 has been studied. Increasing concentrations of 4-chlorophenol (0.4–0.55 mM) were better tolerated and more quickly degraded by the immobilized organisms than by free cells. The capability for haloarene-degradation is inducible. In semicontinuous fermentation at pH 7 a minimal degradation time of 5 h for degrading 0.2 mM 4-chlorophenol was reached. Fermentation temperature was shown to be important for inducing the degradation capability, but to be less important for the degradation rate by induced organisms. High-frequency feeding of small amounts of 4-chlorophenol (0.05 mM) was more favourable than low-frequency feeding of larger amounts (0.15 mM).

Continuous fermentation with unbuffered medium allowed a degradation rate of about 2 mmol·l-1·d-1; with buffered medium a higher degradation rate of nearly 4 mmol·l-1·d-1 was reached, but the Ca-alginate beads dissolved.


Fermentation Biodegradation Degradation Rate Free Cell Continuous Fermentation 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


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Copyright information

© Springer-Verlag 1985

Authors and Affiliations

  • F. Westmeier
    • 1
  • H. J. Rehm
    • 1
  1. 1.Institut für MikrobiologieUniversität MünsterMünsterGermany

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