Abstract
It is shown by the criteria of saturation kinetics, specificity, and inhibition experiments that chromoplast membranes from the daffodil flower contain a phosphate translocator for the counter-exchange of phosphate, and 3-phosphoglycerate, as well as phosphoenolpyruvate; they also contain an adenylate translocator. This is the first report on the occurrence of these translocators in non-green plastids. Both translocators exhibit certain dissimilar properties when compared to the corresponding systems of chloroplasts. The transport rates of both translocators are sufficient to allow a prominent fatty acid synthesis in isolated chromoplasts when C3 intermediates of the glycolytic pathway or adenine nucleotides are used as energy sources.
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Fliege, R., Flügge, U.I., Werdan, K., Heldt, H.W. (1978) Specific transport of inorganic phosphate, 3-phosphoglycerate and triose phosphates across the inner membrane of the envelope in spinach chloroplasts. Biochim. Biophys. Acta502, 232–247
Flügge, U.I., Heldt, H.W. (1978) Specific labelling of the active site of the phosphate translocator in spinach chloroplasts by 2,4,6-trinitrobenzene sulfonate. Biochem. Biophys. Res. Commun.84, 37–44
Hampp, R. (1978) Kinetics of membrane transport during chloroplast development. Plant Physiol.62, 735–740
Heber, U., Santarius, K.A. (1970) Direct and indirect transfer of ATP and ADP across the chloroplast envelope. Z. Naturforsch.25 b, 718–728
Heber, U., Walker, D.A. (1979) The chloroplast envelope-barrier or bridge? TIBS4, 252–256
Heldt, H.W. (1969) Adenine nucleotide translocation in spinach chloroplasts. FEBS Lett.5, 11–14
Heldt, H.W. (1976) Metabolite transport in intact spinach chloroplasts. In: The intact chloroplast, pp. 215–234, Barber, H., ed. Elsevier, Amsterdam
Heldt, H.W., Rapley, L. (1970) Specific transport of inorganic phosphate, 3-phosphoglycerate and dihydroxyacetonephosphate, and of dicarboxylates across the inner membrane of spinach chloroplasts. FEBS Lett.10, 143–148
Heldt, H.W., Sauer, F. (1971) The inner membrane of the chloroplast envelope as the site of specific metabolite transport. Biochim. Biophys. Acta234, 83–91
Kleinig, H., Liedvogel, B. (1978) Fatty acid synthesis by isolated chromoplasts from the daffodil [14C]acetate incorporation and distribution of labelled acids. Eur. J. Biochem.83, 499–505
Kleinig, H., Liedvogel, B. (1980) Fatty acid synthesis by isolated chromoplasts from the daffodil. Energy sources and distribution patterns of the acids. Planta150, 166–169
Krause, G.H., Heber, U. (1976) Energetics in intact chloroplasts. In: The intact chloroplast, pp 171–214, Barber, H., ed. Elsevier, Amsterdam
Liedvogel, B., Kleinig, H. (1979) Galactolipid synthesis in chromoplasts in vitro. Specificities of acyl- and galactosyltransferases. Planta144, 467–471
Liedvogel, B., Sitte, P., Falk, H. (1976) Chromoplasts in the daffodil: fine structure and chemistry. Cytobiologie Z. Exp. Zellforsch.12, 155–174
Mollenhauer, H.H., Kogut, C. (1968) Chromoplast development in daffodil. J. Microsc.7, 1045–1050
Stocking, C.R., Larson, S. (1969) A chloroplast cytoplasmic shuttle and the reduction of extraplastidic NAD. Biochem. Biophys. Res. Commun.37, 278–282
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Liedvogel, B., Kleinig, H. Phosphate translocator and adenylate translocator in chromoplast membranes. Planta 150, 170–173 (1980). https://doi.org/10.1007/BF00582362
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DOI: https://doi.org/10.1007/BF00582362