Advertisement

Pflügers Archiv

, Volume 381, Issue 2, pp 107–111 | Cite as

Calcium inhibits urinary acidification: Effect of the ionophore A23187 on the turtle bladder

  • Jose A. L. Arruda
Transport Processes, Metabolism and Endocrinology; Kidney, Gastrointestinal Tract, and Exocrine Glands

Abstract

The role of intracellular calcium in urinary acidification was studied in the turtle bladder with the use of the ionophore A23187. In the presence of calcium acidification was significantly inhibited in the hemibladders treated with the ionophore as compared to control hemibladders treated with dimethylsulfoxide (the vehicle used to dissolve the inophore). In the absence of calcium both the ionophore and dimethylsulfoxide failed to cause any change in acidification. The apparent proton motive force and active conductance of H+ were unchanged in dimethylsulfoxide treated hemibladders. In the presence of the ionophore and calcium, the proton motive force and the conductance were significantly decreased when the H+ current was low (less than 30% of control values); when the H+ current was decreased, but not less than 30%, the proton motive force was unchanged. These data provide evidence for an important role of intracellular calcium in the regulation of urinary acidification.

Key words

Ionophore A23187 Urinary acidification Turtle bladder 

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. 1.
    Al-Awqati, Q., Mueller, A., Steinmetz, P. R.: Transport of H+ against electrochemical gradients in turtle urinary bladder. Am. J. Physiol.233, F502–508 (1977)Google Scholar
  2. 2.
    Amiel, C., Ardaillou, R., Lecestre, M.: Acidilication de “l” urine apres injection intraveineuse de gluconate de calcium chez “l” homme; III. Etude de “l” excretion des ions HCO3. Rev. Fr. Etud. Clin. Biol.8, 647–652 (1963)Google Scholar
  3. 3.
    Arruda, J. A. L., Dytko, G., Mola, R., Kurtzman, N. A.: Role of magnesium and calcium on transport processes in the turtle bladder. Clin. Res.26, 689 (a) (1978)Google Scholar
  4. 4.
    Borle, A. B.: Calcium metabolism at the cellular level. Fed. Proc.32, 1944–1950 (1973)Google Scholar
  5. 5.
    Case, G. D., Vanderkooi, J. M., Scarpa, A.: Physical properties of biological membranes. determined by the fluorescence of calcium ionophore A23187. Phys. Arch. Biochem. Biophys.162, 174–185 (1974)Google Scholar
  6. 6.
    Crumb, C. K., Martinez-Maldonado, M., Eknoyan, G., Suki, W.: Effects of volume expansion, purified parathyroid extract and calcium on renal bicarbonate absoprtion in the dog. J. Clin. Invest.54, 1287–1293 (1974)Google Scholar
  7. 7.
    Essig, A., Caplan, S. R.: Energetics of active transport processes. Biophys. J.8, 1434–1457 (1968)Google Scholar
  8. 8.
    Karlinsky, M., Sager, D. S., Kurtzman, N. A., Pillay, V. K. G.: Effect of parathormone and cyclic adenosine monophosphate on renal bicarbonate reabsorption. Am. J. Physiol.227, 1226–1231 (1974)Google Scholar
  9. 9.
    Pressman, B. C.: biological applications of the ionophores. Ann. Rev. Biochem.45, 501–526 (1976)Google Scholar
  10. 10.
    Rasmussen, H., Goodman, D. B. P.: Relationships between calcium and cyclic nucleotides in cell activation. Physiol. Rev.57, 421–509 (1977)Google Scholar
  11. 11.
    Reed, P. W., Lardy, H. A.: A23187: A divalent cation ionophore. J. Biol. Chem.247, 6970–6977 (1972)Google Scholar
  12. 12.
    Richet, G., Ardillou, R., Amiel, C., Lecestre, M. L.: Acidification des urines et augmentation de “l” ammoniurie apres injection intraveineuse de gluconate de calcium chez “l” homme. J. Urol. Nephrol.69, 373–398 (1963)Google Scholar
  13. 13.
    Schilb, T. P., Brodsky, W. A.: Acidification of mucosal fluid by transport of bicarbonate ion in turtle bladders. Am. J. Physiol.210, 997–1008 (1966)Google Scholar
  14. 14.
    Schwartz, J. H.: H+ current response to CO2 and carbonic anhydrase inhibition in turtle bladder. Am. J. Physiol.231, 565–572 (1976)Google Scholar
  15. 15.
    Steinmetz, P. R.: Characteristics of hydrogen ion transport in urinary bladder of water turtle. J. Clin. Invest.46, 1531–1540 (1967)Google Scholar
  16. 16.
    Steinmetz, P. R.: Acid-base relations in epithelium of turtle bladder: site of active step in acidification and role of metabolic CO2. J. Clin. Invest.48, 1258–1265 (1969)Google Scholar
  17. 17.
    Steinmetz, P. R.: Cellular mechanisms of urinary acidification. Physiol. Rev.54, 890–956 (1974)Google Scholar
  18. 18.
    Wiesmann, W., Sinha, S., Klahr, S.: Effects of ionophore A23187 on base-line and vasopressin stimulated sodium transport in the toad bladder. J. Clin. Invest.59, 418–425 (1977)Google Scholar

Copyright information

© Springer-Verlag 1979

Authors and Affiliations

  • Jose A. L. Arruda
    • 1
    • 2
  1. 1.Sections of NephrologyUniversity of Illinois Abraham Lincoln School of MedicineUSA
  2. 2.Veterans Administration West Side Hospital in ChicagoUSA

Personalised recommendations