Summary
Initiation factor F3 has been purified fromEscherichia coli and labelledin vitro by reductive alkylation. The14CH3−F3 so obtained had a specific activity of about 1 000 cpm/μg and was shown to have retained its biological activity. Labelled F3 binds to 30S ribosomal subunits ofEscherichia coli andBacillus stearothermophilus, but does not bind to either 70S ribosomes or 50S ribosomal subunits. The stoichiometry of the binding indicates that one molecule of14CH3−F3 is bound to each 30S ribosomal subunit. Several antibiotics, known to interact with 30S subunits, inhibit the binding. Functional studies indicate that F3 is released from 30S ribosomes as a result of the formation of the “70S initiation complex”.
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Communicated by H. G. Wittmann
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Pon, C.L., Friedman, S.M. & Gualerzi, C. Studies on the interaction between ribosomes and14CH3−F3 initation factor. Molec. gen. Genet. 116, 192–198 (1972). https://doi.org/10.1007/BF00582228
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DOI: https://doi.org/10.1007/BF00582228