Summary
Native 30S ribosomal subunits fromEscherichia coli are deficient in fractional protein S21, which is present on the monosome and polysome-derived 30S subunits. The presence of S21 prevents the binding of Fmet-tRNA if and only if 50S subunits are present. In contrast, proteins S2, S3 and S14 stimulate the binding of Fmet-tRNA. These results have been used to rationalize other data concerning the mechanism of Fmet-tRNA binding by ribosomes. In addition, the present data indicate that the 30S ribosomal subunits are heterogeneousin vivo as well asin vitro.
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Communicated by H. G. Wittmann
Dedicated to Professor H. Veldstra on the occasion of his retirement from the chair of biochemistry of the University of Leiden.
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van Duin, J., van Knippenberg, P.H., Dieben, M. et al. Functional heterogeneity of the 30S ribosomal subunit ofEscherichia coli . Molec. gen. Genet. 116, 181–191 (1972). https://doi.org/10.1007/BF00582227
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DOI: https://doi.org/10.1007/BF00582227