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Polymorphismus und Aktivitätsregulation der Esterase-2 beiEphestia kühniella

Polymorphism and regulation of activity of esterase-2 inEphestia kühniella

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Summary

After starch gel electrophoresis, extracts ofEphestia larvae exhibit six esterase bands, which occur in tissue specific patterns. Esterase-2 which is a carboxylesterase (E. C. 3.1.1.1) whows polymorphism consisting of a strong staining slow and a weaker fast migrating band. These bands are autosomally determined by the codominantly manifesting alleles2 mand2 f.Est-2 locus is not linked toa, alb, wa andhe.

The esterase-2 molecule has probably dimer structure, resulting from the third hybrid band observed in heterozygotes and after in vitro hybridization experiments. The mm band is more stable against heat than the ff band. The hybrid fm band shows intermediate heat lability.

In homozygous fat bodies, the mm band stains twice as intensely than the ff band. This may be caused by double activity of theEst-2 mallele or epigenetic regulation. Supposing binomial distribution of the m- and f-subunits this would lead to an activity distribution of mm:mf:ff=43%:43%:14% (type I) in a three banded pattern, a proportion which is exactly found in heterozygous fat bodies. In homozygous testes, however, mm and ff bands are equally expressed and the three bands in heterozygous testes show the expected activity distribution type II of nearly 25%:50%:25%.

It is not known wheather activity distribution type I is caused by epigenetical or genetical regulation. A regulatory gene would be closely linked to the structural gene, because no recombinants with activity distribution type II in the fat body have been found.

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  1. Zoologisches Institut, Abt. für Entwicklungsphysiologie der Universität Tübingen, Tübingen, Deutschland

    Friedrich Leibenguth

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  1. Friedrich Leibenguth
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Leibenguth, F. Polymorphismus und Aktivitätsregulation der Esterase-2 beiEphestia kühniella . Molec. gen. Genet. 116, 166–176 (1972). https://doi.org/10.1007/BF00582225

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  • DOI: https://doi.org/10.1007/BF00582225

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