Summary
Disomic strains ofSaccharomyces cerevisiae were studied by DNA-rRNA hybridization to examine the arrangement of rRNA cistrons on yeast chromosomes as well as to identify a disomic strain which was enriched for rRNA cistrons. Four of the five disomic strains tested showed a per cent hybridization lower than wild type. Two of these strains were found to be disomic for more than one chromosome. A slight increase in the per cent hybridization was observed with DNA isolated from one disomic strain. It was concluded that some chromosomes inSaccharomyces cerevisiae had few if any rRNA cistrons suggesting that the rRNA cistrons are non randomly distributed over the genome. From DNA-tRNA hybridization experiments, evidence for the presence of tyrosine tRNA genes on chromosomes VI was obtained.
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Goldberg, S., Øyen, T., Idriss, J.M. et al. Use of disomic strains to study the arrangement of ribosomal cistrons in saccharomyces of ribosomal cistrons inSaccharomyces cerevisiae . Molec. gen. Genet. 116, 139–157 (1972). https://doi.org/10.1007/BF00582223
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DOI: https://doi.org/10.1007/BF00582223