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Visualization of capillaries in skeletal muscle by the ATPase reaction

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Summary

A simple and reliable technique for the visualization of capillaries in skeletal muscle of dogs, guinea pigs and rats is described. 10–20 μm frozen sections were incubated in a medium containing Ca2+ and ATP following acid preincubation (pH 3.8–4.2). The capillaries stained in black and were readily seen surrounding the muscle fibers. Serial sections were also treated for alkaline phosphatase. Values for capillary density using both methods were not different.

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References

  • Ariano, M. A., Armstrong, R. B., Edgerton, V. R.: Hindlimb muscle fiber populations of five mammals. J. Histochem. Cytochem.21, 51–55 (1973)

    PubMed  CAS  Google Scholar 

  • Banchero, N.: Capilary density of skeletal muscle in dogs exposed to simulated altitude. Proc. Soc. exp. Biol. N. Y.148, 435–439 (1975)

    CAS  Google Scholar 

  • Brooke, M. H., Kaiser, K. K.: Some comments on the histochemical characterization of muscle adenosine triphosphatase. J. Histochem. Cytochem.17, 431–432 (1969)

    PubMed  CAS  Google Scholar 

  • Dubowitz, V., Brooke, M. H.: Muscle biopsy: A modern approach. London-Philadelphia-Toronto: W. B. Saunders 1973

    Google Scholar 

  • Glick, D.: Histochemical localization of adenosine-triphosphatase. Science103, 599 (1946)

    Google Scholar 

  • Gomori, G.: Microtechnical demonstration of phosphatase in tissue sections. Proc. Soc. exp. Biol. (N. Y.)42, 23–26 (1939)

    CAS  Google Scholar 

  • Guth, L., Samaha, F. J.: Qualitative differences between actomyosin ATPase of slow and fast mammalian muscle. Exp. Neurol.25, 138–152 (1969)

    Article  PubMed  CAS  Google Scholar 

  • Heroux, O., St. Pierre, J.: Effect of cold acclimation on vascularization of ears, heart, liver and muscles of white rats. Amer. J. Physiol.188, 163–168 (1957)

    PubMed  CAS  Google Scholar 

  • Krogh, A.: The number and distribution of capillaries in muscle with calculations of the oxygen pressure head necessary for supplying the tissue. J. Physiol. (Lond.)52, 409–415 (1919)

    CAS  Google Scholar 

  • Moog, F., Steinbach, H. B.: Notes on the possibility of a histochemical method for localizing adenosine-triphosphatase. Science103, 144 (1946)

    CAS  Google Scholar 

  • Padykula, H., Herman, E.: The specificity of the histochemical method for adenosine triphosphatase. J. Histochem. Cytochem.3, 170–195 (1955)

    PubMed  CAS  Google Scholar 

  • Plyley, M. J., Groom, A. C.: Geometrical distribution of capillaries in mammalian striated muscle. Amer. J. Physiol.228, 1376–1383 (1975)

    PubMed  CAS  Google Scholar 

  • Romanul, F. C. A.: Capillary supply and metabolism of muscle fibers. Arch. Neurol. (Chic.)12, 497–509 (1965)

    CAS  Google Scholar 

  • Valdivia, E.: Total capillary bed in striated muscle of guinea pigs native to the Peruvian mountains. Amer. J. Physiol.194, 585–589 (1958)

    PubMed  CAS  Google Scholar 

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Author notes

  1. A. H. Sillau

    Present address: IVITA, Universidad de San Marcos, P. O. Box, 4270, Lima, Peru

Authors and Affiliations

  1. Department of Physiology, University of Colorado School of Medicine, 80262, Denver, Colorado, USA

    A. H. Sillau & Natalio Banchero

Authors
  1. A. H. Sillau
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  2. Natalio Banchero
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Additional information

Supported by USPHS NIH Grant HL 12679-HL 18145

Supported by a fellowship from the Rockefeller Fndn

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Sillau, A.H., Banchero, N. Visualization of capillaries in skeletal muscle by the ATPase reaction. Pflugers Arch. 369, 269–271 (1977). https://doi.org/10.1007/BF00582194

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  • DOI: https://doi.org/10.1007/BF00582194

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