Visualization of capillaries in skeletal muscle by the ATPase reaction
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A simple and reliable technique for the visualization of capillaries in skeletal muscle of dogs, guinea pigs and rats is described. 10–20 μm frozen sections were incubated in a medium containing Ca2+ and ATP following acid preincubation (pH 3.8–4.2). The capillaries stained in black and were readily seen surrounding the muscle fibers. Serial sections were also treated for alkaline phosphatase. Values for capillary density using both methods were not different.
Key wordsATPase Capillaries Fiber typification Skeletal muscle
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