The relationship between stimulation-induced potassium release and amylase secretion in the mouse parotid
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The output of amylase from superfused mouse parotid segments in response to stimulation with acetylcholine (ACh), phenylephrine and isoprenaline during exposure to solutions with varying potassium concentrations was monitored by an on line automated fluorometric method.
During stimulation with ACh or phenylephrine a 10-fold increase in superfusion fluid potassium concentration caused an immediate very marked reduction in amylase output which was fully reversible. A 10-fold reduction in potassium concentration resulted in a prominent rise in amylase output. During stimulation with isoprenaline there was no effect on the amylase output of varying the extracellular potassium concentration. Acetylcholine and phenylephrine caused potassium release from the mouse parotid whereas isoprenaline had no such effect.
It appears that under conditions where stimulation-induced potassium release is enhanced there is also an enhanced amylase secretion and vice versa. There may therefore be a link between passive potassium transport and amylase secretion.
Key wordsParotid Amylase secretion Potassium release α- and β-Adrenoceptor Acetylcholine
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- Burgen, A. S. V.: The secretion of potassium in saliva. J. Physiol. (Lond.)132, 20–39 (1956)Google Scholar
- Petersen, O. H.: Some factors influencing stimulation-induced release of potassium from the cat submandibular gland to fluid perfused through the gland. J. Physiol. (Lond.)208, 431–447 (1970a)Google Scholar
- Petersen, O. H.: The importance of extracellular sodium and potassium for acetylcholine-evoked salivary secretion. Experientia (Basel)26, 1103–1104 (1970b)Google Scholar
- Petersen, O. H.: Stimulus-secretion coupling of salivary acinar cells: the role of membrane permeability change, Ca2+ and cyclic AMP. In: Stimulus-secretion coupling in the gastrointestinal tract (R. M. Case and H. Goebell, eds.) pp. 281–293. Lancaster, MTP 1976bGoogle Scholar
- Petersen, O. H.: Increase in membrane conductance by adrenaline in parotid acinar cells. Experientia (Basel)32, 471–472 (1976c)Google Scholar
- Petersen, O. H., Ueda, N.: Pancreatic acinar cells: the role of calcium in stimulus-secretion coupling. J. Physiol. (Lond.)254, 583–606 (1976)Google Scholar
- Selinger, Z., Eimerl, S., Savion, N., Schramm, M.: A Ca2+ ionophore A23187 simulating hormone and neurotransmitter in the rat parotid and pancreas glands. In: Secretory mechanisms of exocrine glands (N. A. Thorn and O. H. Petersen, eds.), pp. 68–87. Copenhagen: Munksgaard 1974Google Scholar