Pflügers Archiv

, Volume 414, Issue 6, pp 634–639 | Cite as

Study on the renal handling of sex dependent proteins in male rats studied by micropuncture techniques and by the isolated perfused rat kidney

  • E. Jonas
  • J. M. Alt
  • H. J. Schurek
  • R. Brunkhorst
  • H. Stolte
Transport Processes, Metabolism and Endocrinology; Kidney, Gastrointestinal Tract, and Exocrine Glands

Abstract

The separation of sex dependent urinary proteins of the rat (SDP) by micro-disc electrophoresis results in at least eight well defined protein bands with differing molecular weights. The hepatic origin of a sex dependent urinary protein, named α2u-globulin, has been demonstrated before by other authors applying immunological methods. In the present study, it could been shown that SDP circulate in the plasma at a concentration of 23.8 mg/l. The origin of those protein bands which appear typically upon electrophoresis was still under dispute because they could not been demonstrated in proximal tubular fluid. The present study confirms the extrarenal source of SDP and suggests identity with α2u-globulin. The attempt to track down SDP from plasma to excreted urine demonstrated that, in contrast to proximal fluid, samples from nephron parts distal to the loop of Henle contain large amounts of SDP. An isolated kidney model was used to determine the sieving coefficient and tubular reabsorption of SDP, obtained from male rat urine. We have found a correlation between the sieving coefficient and the molecular weight of SDP. The sieving coefficient ranged from 0.375 to 0.834. The tubular reabsorption which has been determined with an isolated kidney perfused with albumin and erythrocytes also showed variation with regard to molecular weight and was 61.7%, on average.

Key words

Sex dependent proteinuria Micro-disc electrophoresis Micropuncture technique Isolated perfused rat kidney 

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Copyright information

© Springer-Verlag 1989

Authors and Affiliations

  • E. Jonas
    • 1
  • J. M. Alt
    • 1
  • H. J. Schurek
    • 1
  • R. Brunkhorst
    • 1
  • H. Stolte
    • 1
  1. 1.Division of Nephrology, Department of Internal MedicineMedical School HannoverHannoverFederal Republic of Germany

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