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Lipopolysaccharide is the receptor for kappa phage inSerratia marcescens

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Abstract

Kappa phage active onSerratia marcescens can form plaques on white and red strains with identical efficiencies. To identify the kappa phage receptor, the inactivation of the phage was studied after incubation with several bacterial subcellular fractions. The experiments demonstrated that kappa phage adsorbs to outer membrane fractions of susceptible cells. Proteinase K did not affect the rate of inactivation. Lipopolysaccharide proved to be the primary receptor for kappa phage. Prodigiosin content of the lipopolysaccharide fraction was low.

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References

  • Cho LKN, Lowe JA, Maguire RB & Tsang JC (1987) Relationship of prodigiosin condensing enzyme activity to the biosynthesis of prodigiosin and its precursors inSerratia marcescens. Experientia 43: 397–399

    Google Scholar 

  • Dauenhauer SA, Hull RA & Williams RP (1984) Cloning and expression inEscherichia coli ofSerratia marcescens genes encoding prodigiosin biosynthesis. J. Bacteriol. 158: 1128–1132

    Google Scholar 

  • Ellmauer H & Kaplan RW (1959) Auslösung von klarplaquemutationen durch UV-bestrahlung des freien phagen kappa vonSerratia marcescens. Naturwissenschaften 46: 150

    Google Scholar 

  • Grimont PAD & Grimont F (1984) Genus VIII.Serratia. In: Krieg NR (Ed) Bergey's Manual of Systematic Bacteriology, Vol 1 (pp 477–484). Williams and Wilkins, Baltimore

    Google Scholar 

  • Hancock REW & Nikaido H (1978). Outer membrane of gramnegative bacteria. XIX. Isolation fromPseudomonas aeruginosa PAO1 and use in reconstitution and definition of the permeability barrier. J. Bacteriol. 136: 381–390

    Google Scholar 

  • Laemmli UK (1970) Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature (London) 227: 680–685

    Google Scholar 

  • Morrison DA (1966) Prodigiosin synthesis in mutants ofSerratia marcescens. J. Bacteriol. 91: 1599–1640

    Google Scholar 

  • Paruchuri DK & Harshey RM (1987) Flagellar variation inSerratia marcescens is associated with color variation. J. Bacteriol. 169: 61–65

    Google Scholar 

  • Patel KA, Mehta AM & Dave PJ (1982) Prodigiosin, a component of kappa phage receptor complex inSerratia marcescens. Microbios 34: 153–158

    Google Scholar 

  • Qadri SMH & Williams RP (1973) Role of methionine in biosynthesis of prodigiosin bySerratia marcescens. J. Bacteriol. 116: 1191–1198

    Google Scholar 

  • Qadri SMH & Williams RP (1973) Incorporation of amino acids carbon into prodigiosin synthesized by non-proliferating cells ofSerratia marcescens. Can. J. Microbiol. 20: 461–468

    Google Scholar 

  • Scott RH, Qadri SMH & Williams RP (1976) Role of L-proline in the biosynthesis of prodigiosin. Appl. Environ. Microbiol. 32: 561–566

    Google Scholar 

  • Tomas J & Jofre J (1985) Lipopolysaccharide-specific bacteriophage forKlebsiella pneumoniae C3. J. Bacteriol. 162: 1267–1279

    Google Scholar 

  • Trias J, Viñas M, Guinea J & Lorén JG (1988) Induction of yellow pigmentation inSerratia marcescens. Appl. Environ. Microbiol. 54: 3138–3184

    Google Scholar 

  • —, Viñas M, (1987) A new method for mutant enrichment forSerratia marcescens using the antibiotic azthreonam. Lett. Appl. Microbiol. 4: 81–84

    Google Scholar 

  • —, Viñas M, (1987) Isolation from urine of twoSerratia marcescens strains excreting a diffusible yellow pigment. J. Gen. Microbiol. 133: 773–777

    Google Scholar 

  • —, Viñas M, (1989) Brown pigmentation inSerratia marcescens cultures associated with tyrosine metabolism. Can. J. Microbiol. 35: 1037–1042

    Google Scholar 

  • Tsai CM & Frasch CE (1982) A sensitive silver-stain for detecting lipopolysaccharide in polyacrylamide gels. Anal. Biochem. 119: 115–119

    Google Scholar 

  • Tsang JC & Kallvy DM (1971) Association of prodigiosin with outer cell wall components. Trans. Ill. Acad. Sci. 64: 22–26

    Google Scholar 

  • Viñas M, Lorén JG & Guinea J (1983) Particulate-bound pigment ofSerratia marcescens and its association with the cellular envelopes. Microbios Letters, 24: 19–26

    Google Scholar 

  • Williams RP (1973) Biosynthesis of prodigiosin, a secondary metabolite ofSerratia marcescens Appl. Microbiol. 25: 396–402

    Google Scholar 

  • Williams RP, Gott CL & Qadri SMH (1971) Induction of pigmentation in nonproliferating cells ofSerratia marcescens by addition of single amino acids. J. Bacteriol. 106: 444–448

    Google Scholar 

  • Williams RP & Qadri SMH (1980) The pigment ofSerratia In: vonGraevenitz A & Rubin SJ (Eds) The GenusSerratia (pp 31–75). CRC Press, Inc., Boca Raton, Fla

    Google Scholar 

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Montilla, R., Williams, R.P., Lorén, J.G. et al. Lipopolysaccharide is the receptor for kappa phage inSerratia marcescens . Antonie van Leeuwenhoek 59, 15–18 (1991). https://doi.org/10.1007/BF00582114

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  • DOI: https://doi.org/10.1007/BF00582114

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