Summary
Ooplasmic segregation inPhallusia mammillata was completed 3 to 5 min after fertilization. Colchicine, which completely stopped nuclear divisions, did not inhibit segregation. Cytochalasin B, which prevented cleavage at a low concentration (0.2 μg/ml) inhibited segregation only at a concentration at least five times higher. The action of these drugs leads to the conclusion that ooplasmic segregation does not depend on an assembly of microtubules or on microfilaments which are involved in cell division.
This is a preview of subscription content, log in via an institution to check access.
References
Borisy, G. C., Taylor, E. W.: The mechanism of action of Colchicine. J. Cell Biol.34, 525–548 (1967)
Clement, A. C.: Development of the vegetal half of theIlyanassa egg after removal of most of the yolk by centrifugal force, compared with the development of animal halves of similar visible composition. Develop. Biol.17, 165–186 (1968)
Conklin, E. G.: The organization and cell-lineage of the ascidian egg. J. Acad. nat. Sci. Philad.13, 1–119 (1905)
Estensen, R. D., Plagemann, P. G. W.: Cytochalasin B: inhibition of glucose and glucosamine transport. Proc. nat. Acad. Sci. (Wash.)69, 1430–1431 (1972)
Everhart, Jr., L. P., Rubin, R. W.: Cyclic changes in the cell surface. I. Change in thymidine transport and its inhibition by cytochalasin B in Chinese hamster ovary cells. J. Cell Biol.60, 434–441 (1974)
Freed, J. J., Lebowitz, M. M.: The association of a class of saltating movements with microtubules in cultured cells. J. Cell Biol.45, 334–354 (1970)
La Spina, R.: Lo spostamento dei plasmi mediante centrifugazione nell uovo vergine di Ascidie e il consequente sviluppo. Acta Embryol. Morphol. exp. (Palermo)3, 66–78 1958)
MacGregor, H. C., Stebbings, H.: A massive system of microtubules associated with cytoplasmic movement in telotrophic ovarioles. J. Cell Sci.6, 431–449 (1970)
Nelson, D. R., Jaffe, L. F.: Cells without cytoplasmic movements respond to cytochalasin. Develop. Biol.30, 206–208 (1973)
Porter, K. R.: Cytoplasmic microtubules and their function. In: Principles of biomolecular organization. Ciba Foundation Symposium (G. W. W. Wolstenholme and M. O'Connor, eds.). London: J. & A. Churchill Ltd. 1966
Quantrano, R. S.: Separation of processes associated with differentiation of two-celled Fucus embryos. Develop. Biol.30, 209–213 (1973)
Raven, Ch. P.: The cortical and subcortical cytoplasm of theLymnaea egg. Int. Rev. Cytol.28, 1–44 (1970)
Reverberi, G.: The mitochondrial pattern in the development of the Ascidian egg. Experientia (Basel)12, 55–60 (1956)
Reverberi, G.: Ascidia. In: Experimental embryology of marine and fresh water invertebrates (G. Reverberi, ed.). Amsterdam: North Holland Publ. Co. 1971
Raff, R. A.: Polar lobe formation by embryos ofIlyanassa obsoleta. Effects of inhibitors of microtubule and microfilament function. Exp. Cell Res.71, 455–457 (1972)
Spooner, B. S.: Cytochalasin B: Toward an understanding of its mode of action. Develop. Biol.35, f. 13-f. 18 (1973)
Szolossi, D.: Cortical cytoplasmio filaments of cleaving eggs: a structural element corresponding to the contractile ring. J. Cell Biol.44, 192–209 (1970)
Wessells, H. K., Spooners, B. S., Ash, J. F., Bradley, M. O., Luduena, M. A., Taylor, E. L., Wrenn, K. J. T., Yamada, K. M.: Microfilaments in cellular and developmental processes. Science171, 135–143 (1971)
Whittaker, J. R.: Differenciation without cleavage in an ascidian egg: development of muscle acetylcholinesterase. Biol. Bull.141, 407–408 (1971)
Author information
Authors and Affiliations
Additional information
This work was performed at the Station Zoologique Villefranche-sur mer (Director: Prof. P. Bougis). The work was supported by a grant A.T.P. of C.N.R.S.
Rights and permissions
About this article
Cite this article
Zalokar, M. Effect of Colchicine and cytochalasin B on ooplasmic segregation of ascidian eggs. W. Roux' Archiv f. Entwicklungsmechanik 175, 243–248 (1974). https://doi.org/10.1007/BF00582094
Received:
Issue Date:
DOI: https://doi.org/10.1007/BF00582094