Summary
The addition of excess orotic acid to cultures of normal human lymphocytes stimulated with phytohaemagglutinin causes a reduction in the mitotic rate. It is suggested that this is caused by competition for the available 5-phosphoribosyl-1-pyrophosphate in the cell leading to a reduced purine synthesis. The significance of these results with regard to orotic aciduria is discussed.
Zusammenfassung
Zugabe von Orotsäure im überschuß zu Kulturen normaler menschlicher Lymphocyten, die mit Phytohämagglutinin stimuliert wurden, bewirkt eine Reduktion der Mitoserate. Es wird vorgeschlagen, daß dies die Folge einer Kompetition für das vorhandene 5-Phosphoribosyl-1-pyrophosphat ist, welche zu einer reduzierten Purinsynthese fü hrt
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References
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I wish to thank the Smith Kline and French Foundation for financial support and Miss B. Wilson for technical assistance.
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Timson, J. Effect of orotic acid on the mitosis of phytohaemagglutinin stimulated human lymphocytes in culture. W. Roux' Archiv f. Entwicklungsmechanik 164, 199–200 (1969). https://doi.org/10.1007/BF00581999
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DOI: https://doi.org/10.1007/BF00581999