Species-specific responses of muscle lactate dehydrogenase isozymes to increased contractile activity

  • Jean-Aimé Simoneau
  • Dirk Pette
Heart, Circulation, Respiration and Blood; Environmental and Exercise Physiology Short Communication


Analyses of lactate dehydrogenase (LDH) isozymes were performed in normal and chronically stimulated (10 Hz, 10 h/d) tibialis anterior (TA) muscles of the mouse, rat, guinea pig, and rabbit. The electrophoretic mobilities of the individual LDH isozymes of normal muscles revealed speciesspecific patterns. Stimulation up to 28 days evoked increases in the percentage of the H-subunit of LDH. The absolute increase was highest in the rabbit, intermediate in the guinea pig and rat, and lowest in the mouse. The extent of the M- to H-subunit exchange, thus, appeared to be inversely correlated with the basal aerobic-oxidative capacities of the investigated muscles. In addition, a relationship between the expression of the H-subunit or LDH and the increase in aerobic-oxidative capacity became evident from these stimulation-induced processes of metabolic adaptation.

Key words

Chronic, low-frequency nerve stimulation Fast-twitch skeletal muscle Lactate dehydrogenase isozymes Species differences 


  1. Dawson DM, Goodfriend TL, Kaplan NO (1964) Lactic dehydrogenases: Functions of the two types. Science 143:929–933CrossRefPubMedGoogle Scholar
  2. Leberer E, Pette D (1984) Lactate dehydrogenase isozymes in type I, IIA and IIB fibres of rabbit skeletal muscles. Histochemistry 80:295–298CrossRefPubMedGoogle Scholar
  3. Pette D, Smith ME, Staudte HW, Vrbová G (1973) Effects of long-term electrical stimulation on some contractile and metabolic characteristics of fast rabbit muscles. Pflügers Arch 338:257–272CrossRefPubMedGoogle Scholar
  4. Plagemann PGW, Gregory KF, Wroblewski F (1960) The electrophoretically distinct forms of mammalian dehydrogenase. J Biol Chem 235:2282–2287PubMedGoogle Scholar
  5. Seedorf U, Leberer E, Kirschbaum BJ, Pette D (1986) Neural control of gene expression in skeletal muscle. Effects of chronic stimulation upon lactate dehydrogenase isozymes and citrate synthase. Biochem J 239:115–120PubMedGoogle Scholar
  6. Simoneau J-A, Pette D (1988) Species-specific effects of chronic nerve stimulation upon tibialis anterior muscle in mouse, rat, guinea pig, and rabbit. Pflügers Arch 412:86–92PubMedGoogle Scholar
  7. Thorling EB, Jensen K (1966) The lactate dehydrogenase isoenzymes in various organs of the rabbit in anemia, hypoxia, and after cobalt administration. Acta Pathol Microbiol Scand 66:426–436PubMedGoogle Scholar

Copyright information

© Springer-Verlag 1989

Authors and Affiliations

  • Jean-Aimé Simoneau
    • 1
  • Dirk Pette
    • 1
  1. 1.Fakultät für BiologieUniversität KonstanzKonstanzFederal Republic of Germany

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