Heart, Circulation, Respiration and Blood; Environmental and Exercise Physiology Short Communication
Analyses of lactate dehydrogenase (LDH) isozymes were performed in normal and chronically stimulated (10 Hz, 10 h/d) tibialis anterior (TA) muscles of the mouse, rat, guinea pig, and rabbit. The electrophoretic mobilities of the individual LDH isozymes of normal muscles revealed speciesspecific patterns. Stimulation up to 28 days evoked increases in the percentage of the H-subunit of LDH. The absolute increase was highest in the rabbit, intermediate in the guinea pig and rat, and lowest in the mouse. The extent of the M- to H-subunit exchange, thus, appeared to be inversely correlated with the basal aerobic-oxidative capacities of the investigated muscles. In addition, a relationship between the expression of the H-subunit or LDH and the increase in aerobic-oxidative capacity became evident from these stimulation-induced processes of metabolic adaptation.
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Dawson DM, Goodfriend TL, Kaplan NO (1964) Lactic dehydrogenases: Functions of the two types. Science 143:929–933CrossRefPubMedGoogle Scholar
Leberer E, Pette D (1984) Lactate dehydrogenase isozymes in type I, IIA and IIB fibres of rabbit skeletal muscles. Histochemistry 80:295–298CrossRefPubMedGoogle Scholar
Pette D, Smith ME, Staudte HW, Vrbová G (1973) Effects of long-term electrical stimulation on some contractile and metabolic characteristics of fast rabbit muscles. Pflügers Arch 338:257–272CrossRefPubMedGoogle Scholar
Plagemann PGW, Gregory KF, Wroblewski F (1960) The electrophoretically distinct forms of mammalian dehydrogenase. J Biol Chem 235:2282–2287PubMedGoogle Scholar
Seedorf U, Leberer E, Kirschbaum BJ, Pette D (1986) Neural control of gene expression in skeletal muscle. Effects of chronic stimulation upon lactate dehydrogenase isozymes and citrate synthase. Biochem J 239:115–120PubMedGoogle Scholar
Simoneau J-A, Pette D (1988) Species-specific effects of chronic nerve stimulation upon tibialis anterior muscle in mouse, rat, guinea pig, and rabbit. Pflügers Arch 412:86–92PubMedGoogle Scholar
Thorling EB, Jensen K (1966) The lactate dehydrogenase isoenzymes in various organs of the rabbit in anemia, hypoxia, and after cobalt administration. Acta Pathol Microbiol Scand 66:426–436PubMedGoogle Scholar