Pflügers Archiv

, Volume 405, Supplement 1, pp S110–S114 | Cite as

Relationship between luminal Na+/H+ exchange and luminal K+ conductance in diluting segment of frog kidney

  • H. Oberleithner
  • P. Dietl
  • G. Münich
  • M. Weigt
  • A. Schwab
Na+/H+ Exchange

Abstract

Experiments were performed in the isolated perfused kidney of K+ adaptedRana pipiens to investigate the relationship between luminal K+ conductance and H+ transport in cells of the diluting segment. Inhibition of luminal Na+/H+ exchange by amiloride or by omission of luminal Na+ blocked luminal K+ conductance. Acidification of the kidney perfusate by elevation of pCO2 also reduced luminal K+ conductance. This effect could be prevented by furosemide. Since the steepest transcellular Na+ potential difference, directed from the lumen into the cell, is found when luminal Na+/Cl/K+ cotransport is inhibited by furosemide, we conclude that luminal Na+/H+ exchange is most efficient at these conditions and thus could attenuate intracellular acidification.

Key words

Diluting segment Furosemide K+-conductance Amiloride 

References

  1. 1.
    Balaban RS, Mandel LJ, Benos DJ (1979) On the cross-reactivity of amiloride and 2,4,6 triaminopyrimidine (TAP) for the cellular entry and tight junctional cation permeation pathways in epithelia. J Membr Biol 49:363–390Google Scholar
  2. 2.
    Burg M, Good D (1983) Sodium chloride coupled transport in mammalian nephrons. Annu Rev Physiol 45:533–547Google Scholar
  3. 3.
    Greger R (1985) Ion transport mechanisms in the thick ascending limb of Henle's loop of the mammalian nephron. Physiol Rev (in press)Google Scholar
  4. 4.
    Greger R, Schlatter E (1983a) Properties of the lumen membrane of the cortical thick ascending limb of Henle's loop of rabbit kidney. Pflügers Arch 396:325–334Google Scholar
  5. 5.
    Greger R, Schlatter E (1983b) Properties of the basolateral membrane of the cortical thick ascending of Henle's loop of rabbit kidney. A model for secondary active chloride transport. Pflügers Arch 396:315–324Google Scholar
  6. 6.
    Guggino WB, Stanton BA, Giebisch G (1982) Regulation of apical potassium conductance in the isolated early distal tubule of the Amphiuma kidney. Biophys J 37:338Google Scholar
  7. 7.
    Guggino WB, Oberleithner H, Giebisch G (1984) Relationship between cell volume and ion transport in the early distal tubule of the amphiuma kidney. J Gen Physiol (in press)Google Scholar
  8. 8.
    Herbert SC, Andreoli TE (1984) Control of NaCl transport in the thick ascending limb. Am J Physiol 246,15:F745–F756Google Scholar
  9. 9.
    Oberleithner H (1985) Intracellular pH in diluting segment of frog kidney. Pflügers Arch (in press)Google Scholar
  10. 10.
    Oberleithner H, Guggino W, Giebisch G (1982) Mechanism of distal tubular chloride transport in Amphiuma kidney. Am J Physiol 242:F33-F339Google Scholar
  11. 11.
    Oberleithner H, Lang F, Greger R, Wang W, Giebisch G (1983) Effect of luminal potassium on cellular sodium activity in the early distal tubule of Amphiuma kidney. Pflügers Arch 396:34–40Google Scholar
  12. 12.
    Oberleithner H, Lang F, Wang W, Messner G, Deetjen P (1983) Evidence for an amiloride sensitive Na+ pathway in the amphibian diluting segment induced by K+ adaptation. Pflügers Arch 399:166–172Google Scholar
  13. 13.
    Oberleithner H, Ritter M, Lang F, Guggino W (1983) Anthracene-9-carboxylic acid inhibits renal chloride reabsorption. Pflügers Arch 398:172–174Google Scholar
  14. 14.
    Oberleithner H, Lang F, Messner G, Wang W (1984) Mechanism of hydrogen ion transport in the diluting segment of frog kidney. Pflügers Arch 402:272–280Google Scholar
  15. 15.
    Stoner LC (1977) Isolated perfused amphibian renal tubules: the diluting segment. Am J Physiol 2323:F438-F444Google Scholar

Copyright information

© Springer-Verlag 1985

Authors and Affiliations

  • H. Oberleithner
    • 1
  • P. Dietl
    • 1
  • G. Münich
    • 1
  • M. Weigt
    • 1
  • A. Schwab
    • 1
  1. 1.Institut für PhysiologieUniversität WürzburgWürzburgFederal Republic of Germany

Personalised recommendations