Pflügers Archiv

, Volume 405, Supplement 1, pp S110–S114 | Cite as

Relationship between luminal Na+/H+ exchange and luminal K+ conductance in diluting segment of frog kidney

  • H. Oberleithner
  • P. Dietl
  • G. Münich
  • M. Weigt
  • A. Schwab
Na+/H+ Exchange


Experiments were performed in the isolated perfused kidney of K+ adaptedRana pipiens to investigate the relationship between luminal K+ conductance and H+ transport in cells of the diluting segment. Inhibition of luminal Na+/H+ exchange by amiloride or by omission of luminal Na+ blocked luminal K+ conductance. Acidification of the kidney perfusate by elevation of pCO2 also reduced luminal K+ conductance. This effect could be prevented by furosemide. Since the steepest transcellular Na+ potential difference, directed from the lumen into the cell, is found when luminal Na+/Cl/K+ cotransport is inhibited by furosemide, we conclude that luminal Na+/H+ exchange is most efficient at these conditions and thus could attenuate intracellular acidification.

Key words

Diluting segment Furosemide K+-conductance Amiloride 


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Copyright information

© Springer-Verlag 1985

Authors and Affiliations

  • H. Oberleithner
    • 1
  • P. Dietl
    • 1
  • G. Münich
    • 1
  • M. Weigt
    • 1
  • A. Schwab
    • 1
  1. 1.Institut für PhysiologieUniversität WürzburgWürzburgFederal Republic of Germany

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