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Archives of Dermatological Research

, Volume 255, Issue 1, pp 1–8 | Cite as

Evidence for the involvement of vasoactive constituents of mast cells in axon reflex vasodilatation in the skin of the rat

  • J. A. Kiernan
Article

Summary

Axon reflex vasodilatation following injury to the skin of the pinna of the ear was studied in rats by a thermometric method. Post-traumatic vasodilatation did not occur in animals treated with tyrosine ethyl ester, an inhibitor of chymotrypsin. Vasodilatation was not affected by treatment of the rats with chlorpheniramine (antihistamine) or cyproheptadine (antihistamine and antiserotinin) or with aprotinin, soybean trypsin inhibitor,ɛ-aminocaproic acid or tosyl arginine methyl ester (inhibitors of trypsin and of some other proteinases). Taken in conjunction with the results of other investigations, these findings indicate that in the skin of the rat: (a) histamine and serotinin are not essential for the initiation of axon reflexes, and (b) the chymotrypsin-like proteinase of mast cell granules, released as the result of antidromic activity in sensory axons, may act as a kininogenase and be responsible for causing dilatation of arterioles at the efferent limb of the axon reflex.

Keywords

Mast Cell Ethyl Ester Chymotrypsin Tosyl Soybean Trypsin Inhibitor 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Zusammenfassung

Die durch den Axonreflex bedingte Vasodilatation nach Verletzung der Haut der Ohrmuschel bei Ratten wurde mittels thermometrischer Methoden untersucht. Eine posttraumatische Vasodilatation auf diesem Wege erfolgte nicht bei den Tieren, die mit Tyrosinäthylester, ein Chymotrypsininhibitor, vorbehandelt waren. Die Vasodilatation wurde nicht durch Vorbehandlung der Ratten mit Antihistaminen, Aprotinin, dem Sojabohnentrypsininhibitor ɛ-aminocapronsäure oder Tosylargininmethylester, ein Inhibitor des Trypsins und andere Proteinasen beeinflußt.

In Anbetracht dieser Situation ist die Aussage gestattet, daß in der Rattenhaut Histamin und Serotonin nicht für die Entwicklung des Axonreflexes von Bedeutung sind, daß chymotrypsinähnliche Proteinasen der Mastzellgranula, die durch antidrome Effekte der sensorischen Nerven freigesetzt wurden als Kininogenasen wirken, die für die Dilatation der Arteriolen beim Axonreflex von Wichtigkeit sein dürften.

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References

  1. Archer, G. T.: Release of 5-hydroxytryptamine from rat mast cells in vitro. Nature (Lond.)190, 350 (1961)Google Scholar
  2. Armstrong, D., Dry, R. M. L., Keele, C. A., Markham, J. W.: Observations on chemical excitants of cutancous pain in man. J. Physiol.120, 326–351 (1953)Google Scholar
  3. Auditore, J. V., Ende, N., Katayama, Y.: Multiple character of canine mast cell proteolytic activity. Life Sci.2, 375–381 (1963)Google Scholar
  4. Barnes, C. D., Eltherington, L. G.: Drug Dosage in Laboratory Animals: A Handbook. Revised and enlarged ed. Berkeley, Los Angeles and London: University of California Press 1973Google Scholar
  5. Benditt, E. P., Arase, M.: An enzyme in mast cells with properties like chymotrypsin. J. exp. Med.110, 451–460 (1959)Google Scholar
  6. Bertelli, A.: Proteases and antiproteasic substances in the inflammatory response. Biochem. Pharmacol.17, (Suppl.), 229–240 (1968)Google Scholar
  7. Boyer, P. D. (editor): The Enzymes. Third ed., vol. 3: Hydrolysis: peptide bonds. New York and London: Academic Press 1971Google Scholar
  8. Chapman, L. F., Goodell, H., Wolff, H. G.: Structures and processes involved in the sensation of itch. In: Advances in the Biology of Skin, ed. W. Montagna, pp 161–188. Oxford: Pergamon Press 1960Google Scholar
  9. Chapman, L. F., Ramos, A. O., Goodell, H., Wolff, H. G.: Neurohumoral features of afferent fibers in man. Arch. Neurol.4, 617–650 (1961)Google Scholar
  10. Fernex, M.: The Mast-Cell System. Baltimore: Williams and Wilkins 1968Google Scholar
  11. Folkow, B.: The nervous control of the blood vessels. In: The Control of the Circulation of the Blood, ed. R. J. S. McDowell. Suppl. Vol. pp 1–85. London: Dawson 1956Google Scholar
  12. Folkow, B., Neil, E.: Circulation. New York-London-Toronto: Oxford University Press 1971Google Scholar
  13. Holton, P.: The liberation of adenosine triphosphate on antidromic stimulation of sensory nerves. J. Physiol.145, 494–504 (1959)Google Scholar
  14. Holton, P., Perry, W. L. M.: On the transmitter responsible for antidromic vasodilatation in the rabbit's ear. J. Physiol.114, 240–251 (1951)Google Scholar
  15. Kiernan, J. A.: Degranulation of mast cells following antidromic stimulation of cutaneous nerves. J. Anat.111, 349–350 (1971)Google Scholar
  16. Kiernan, J. A.: The involvement of mast cells in vasodilatation due to axon reflexes in injured skin. Quart. J. exp. Physiol.57, 311–317 (1972a)Google Scholar
  17. Kiernan, J. A.: Effects of known and suspected neurotransmitter substances and of some nucleotides on isolated mast cells. Experientia (Basel)28, 653–655 (1972b)Google Scholar
  18. Kiernan, J. A.: Action of adenosine triphosphate on mast cells in normal and denervated skin. Arch. Derm. Forsch.251, 83–86 (1974)Google Scholar
  19. Kiernan, J. A.: A pharmacological and histological investigation of the involvement of mast cells in cutaneous axon reflex vasodilatation. Quart. J. exp. Physiol.60, 123–130 (1975)Google Scholar
  20. Labelle, A., Tislow, R.: Studies on prophenpyridamine (Trimeton) and chlorophenpyridamine (Chlortrimeton). J. Pharmacol. exp. Ther.113, 72–88 (1955)Google Scholar
  21. Lagunoff, D.: The properties of mast cell proteases. Biochem. Pharmacol.17, (Suppl.), 221–227 (1968)Google Scholar
  22. Lagunoff, D., Benditt, E. P.: Histochemical examinations of chymotrypsin-like esterases. Nature (Lond.)192, 1198–1199 (1961)Google Scholar
  23. Lagunoff, D., Benditt, E. P.: Benzosalicylanilide substrates of proteolytic enzymes. Kinetic and histochemical studies. I. Chymotrypsin. Biochemistry3, 1427–1431 (1964)Google Scholar
  24. Lewis, T.: The Blood Vessels of the Human Skin and their Responses. London: Shaw 1927Google Scholar
  25. Parratt, J. R., West, G. B.: Release of 5-hydroxytryptamine and histamine from tissues of the rat. J. Physiol.137, 179–192 (1957)Google Scholar
  26. Riley, J. F.: The Mast Cells. Edinburgh and London: Livingstone 1959Google Scholar
  27. Rocha e Silva, M.: Kinin Hormones. Springfield, Ill.: Thomas 1970Google Scholar
  28. Rowley, D. A., Benditt, E. P.: 5-hydroxytryptamine and histamine as mediators of the vascular injury produced by agents which damage mast cells in rats. J. exp. Med.103, 399–412 (1956)Google Scholar
  29. Ryan, T. J.: Measurement of blood flow and other properties of the vessels of the skin. In: The Physiology and Pathophysiology of the Skin; vol. 2, The nerves and blood vessels. Ch. 17, pp 654–679. Ed. A. Jarrett. London and New York: Academic Press 1973Google Scholar
  30. Schachter, M.: Kallikreins and kinins. Physiol. Rev.49, 509–547 (1969)Google Scholar
  31. Selye, H.: The Mast Cells. Washington: Butterworths 1965Google Scholar
  32. Selye, H., Gabbiani, G., Tuchweber, B.: The effect of cyproheptadine on the anaphylactoid reaction and the localization of certain calciphylactic syndromes. Ann. Allergy20, 777–788 (1962)Google Scholar
  33. Stone, C. A., Wengler, H. C., Ludden, C. T., Stavorski, J. M., Ross, C. A.: Antiserotonin-antihistaminic properties of cyrpoheptadine. J. Pharmacol. exp. Ther.131, 73–84 (1961)Google Scholar
  34. Vogel, R.: Trautschold, I., Werle, E.: Natural Proteinase Inhibitors. New York: Academic Press 1968Google Scholar
  35. Weiner, R., Zweifach, B. W.: Vasoinhibitory action of proteolytic enzyme inhibitors in the capillary bed. Amer. J. Physiol.211, 725–729 (1966)Google Scholar
  36. Wilhelm, D. L.: Chemical mediators. In: The Inflammatory Process, Second ed. vol. 2, Ch. 8, pp 251–301. Ed. B. W. Zweifach, L. Grant and R. T. McCluskey. New York and London: Academic Press 1973Google Scholar
  37. Yamasaki, H., Saeki, K.: Inhibition of mast cell degranulation by anti-inflammatory agents. Arch. int. Pharmacodyn.168, 166–179 (1967)Google Scholar
  38. Zar, J. H.: Biostatistical Analysis. Englewood Cliffs, N. J.: Prentice-Hall 1974Google Scholar
  39. Zweifach, B. W., Nagler, A. L., Troll, W.: Some effects of proteolytic inhibitors on tissue injury and systemic anaphylaxis. J. exp. Med.113, 437–450 (1961)Google Scholar

Copyright information

© Springer-Verlag 1976

Authors and Affiliations

  • J. A. Kiernan
    • 1
  1. 1.Department of Anatomy, Health Sciences CentreThe University of Western OntarioLondon, OntarioCanada

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