Pflügers Archiv

, Volume 400, Issue 3, pp 241–249 | Cite as

Secretion and contraluminal uptake of dicarboxylic acids in the proximal convolution of rat kidney

  • K. J. Ullrich
  • H. Fasold
  • G. Rumrich
  • S. Klöss
Transport Processes, Metabolism and Endocrinology; Kidney, Gastrointestinal Tract, and Exocrine Glands

Abstract

The transport of dicarboxylic acids in the proximal convolution was investigated by measuring: a) the zero net flux transtubular concentration difference ofdl-methyl-succinate, b) its 2-s influx from the interstitium into tubular cells, and c) its 3.5-s efflux from the tubular lumen. With the first method a luminal concentration exceeding the peritubular concentration was observed, thus indicating a net active transtubular secretion of this slowly metabolized substance.

All transport steps, luminal and contraluminal, as well as the overall transport, were Na+-dependent and inhibited by lithium (apparentKi ≈ 1.8 mmol/l). The overall transport of methylsuccinate, as well as the contraluminal influx into proximal tubular cells, could be inhibited by paraaminohippurate and H2-DIDS with an apparentKi of ≈ 1.8 mmol/l, by taurocholate with an apparentKi ≈ 3.` mmol/l and by pyruvate with an apparentKi ≈ 5 mmol/l, but not by sulfate, thiosulfate,l-lactate, oxalate and urate. As judged from the inhibition of contraluminal methylsuccinate influx by 48 dicarboxylic acids (aliphatic and aromatic), a specificity pattern was observed similar to that of inhibition of luminal efflux of 2-oxoglutarate [22]: a preference of dicarboxylates in the transconfiguration with a chain length of 4–5 carbons; little change in the inhibitory potency with CH3, OH−, SH−and O=, but strong reduction with a NH 3 + in the 2 position; only a small reduction of inhibitory potency with 2,3 disubstituted SH and OH analogs; preference of the dicarboxylic benzene in the 1,4 position and of the diacetyl benzene in the 1,2 position. The data indicate a Na+-dependent dicarboxylic transport system at the contraluminal cell side of the proximal tubule which is very similar to the luminal transport system for dicarboxylic acids.

Key words

Basolateral cell membrane Methylsuccinate-transport 2-Oxolutarate-transport Citrate-transport Lithium 

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Copyright information

© Springer-Verlag 1984

Authors and Affiliations

  • K. J. Ullrich
    • 1
  • H. Fasold
    • 2
  • G. Rumrich
    • 1
  • S. Klöss
    • 1
  1. 1.Max-Planck-Institut für BiophysikFrankfurt/Main 70Federal Republic of Germany
  2. 2.Institut für Biochemie der J. W. Goethe-UniversitätFrankfurt/Main 70Federal Republic of Germany

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