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A high-conductance anion channel in adult amphibian skeletal muscle

  • Excitable Tissues and Central Nervous Physiology
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Abstract

Membrane patches were excised from enzymatically dissocfated frog toe muscle. High-conductance anion channels could be induced in previously quiet patches by 20–120 s depolarizations beyond +20 mV and then studied in the potential range from −80 to +60 mV for a long time. From reversal potentials the estimated permeability ratiosP Cl/P Na andP Cl/P glucuronate were near 3.5 and 4, respectively. There were probably 5 or more conductance levels (substates) for a single channel, the most common in symmetrical 110 mM NaCl being 260 and 70 pS at 10°C. Gating was complex, with rapid and slow events and several gating modes, including periods of rapid flickering. Channels closed reversibly at potentials more negative than −50 mV. The channel was blocked by application to the cytoplasmic face of tannic acid, gallic acid, and zinc but not of DIDS or 9-anthracene-carboxylic acid, and it was blocked by extracellular zinc.

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Authors and Affiliations

  1. Department of Physiology and Biophysics, University of Washington, School of Medicine, 98195, Seattle, WA, USA

    Karl H. Woll, Mark D. Leibowitz & Bertil Hille

  2. I. Physiologisches Institut der Universität des Saarlandes, D-6650, Homburg/Saar, Federal Republic of Germany

    Karl H. Woll & Berthold Neumcke

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  1. Karl H. Woll
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  2. Mark D. Leibowitz
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  3. Berthold Neumcke
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  4. Bertil Hille
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Woll, K.H., Leibowitz, M.D., Neumcke, B. et al. A high-conductance anion channel in adult amphibian skeletal muscle. Pflugers Arch. 410, 632–640 (1987). https://doi.org/10.1007/BF00581324

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  • DOI: https://doi.org/10.1007/BF00581324

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