Pflügers Archiv

, Volume 413, Issue 1, pp 38–42 | Cite as

Neuropeptide Y antagonises secretagogue evoked chloride transport in rat jejunal epithelium

  • H. M. Cox
  • A. W. Cuthbert
Transport Processes, Metabolism and Endocrinology; Kidney, Gastrointestinal Tract, and Exocrine Glands


Neuropeptide Y (NPY) inhibits electrogenic Cl secretion in rat jejunal epithelium under voltage clamp conditions. This effect is dependent upon endogenous eicosanoid formation since it is blocked by the cyclooxygenase inhibitor, piroxicam, which itself has an inhibitory action upon chloride secretion. A number of chloride secretagogues have been examined for their ability to restore the antisecretory effects of NPY. Data presented here shows that NPY responsiveness is restored, in piroxicam pretreated tissues, by vasoactive intestinal polypeptide (VIP), forskolin, prostaglandin E2 (PGE2) isobutyl-1-methyl-xanthine (IBMX) and dibutyryl cAMP added prior to the neuropeptide. While all these agents cause chloride secretion by elevating intracellular cAMP, NPY is also effective in inhibiting the secretory effects of carbachol (CCh) and substance P (SP), agents believed to act by raising intracellular calcium (Cai). Although there is evidence that NPY can inhibit adenylate cyclase, its ability to attenuate chloride secretion brought about by secretagogues acting through both adenylate cyclase and calcium mechanisms, implies that NPY has either a more general fundamental mechanism or has multiple interactions with different second messenger systems.

Key words

Neuropeptide Y Vasoactive intestinal polypeptide Substance P Gastrointestinal epithelia 


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Copyright information

© Springer-Verlag 1988

Authors and Affiliations

  • H. M. Cox
    • 1
  • A. W. Cuthbert
    • 1
  1. 1.Department of PharmacologyUniversity of CambridgeCambridgeUK

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