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Effects of bradykinin on electrical properties of Madin-Darby canine kidney epithelioid cells

  • Transport Processes, Metabolism and Endocrinology; Kidney, Gastrointestinal Tract, and Exocrine Glands
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Abstract

In the present study we have investigated the influence of bradykinin on the potential difference across the cell membrane (PD) of Madin Darby Canine Kidney (MDCK)-cells. In the absence of bradykinin PD averages −52.6±0.9 mV (n=52). Increasing extracellular potassium concentration from 5.4 to 10 and 20 mmol/l depolarizes the cell membrane by +5.2±0.3 mV (n=8) and +14.9±1.0 mV (n=9), respectively. The application of 0.1 μmol/l bradykinin leads to a transient hyperpolarization of the cell membrane to −70.3±0.6 mV (n=30). During this transient hyperpolarization increasing extracellular potassium concentration from 5.4 to 10 and 20 mmol/l depolarizes the cell membrane by +10.4±0.7 mV (n=10) and +29.2±0.8 mV (n=8) respectively. Application of fragments of bradykinin (0.1 μmol/l) are without significant effect on the potential difference across the cell membrane. 1 mmol/l barium depolarizes the cell membrane by +15.8±1.2 mV (n=9) and abolishes the effect of step increase of extracellular potassium concentration from 5.4 to 10 mmol/l. In the presence of barium, bradykinin leads to a transient hyperpolarization by −24.7±1.3 mV (n=7). During this transient hyperpolarization, the cell membrane is sensitive to extracellular potassium concentration despite the continued presence of barium. In the nominal absence of extracellular calcium, bradykinin leads to a transient hyperpolarization, which can be elicited only once. The transient hyperpolarization is not affected by the presence of verapamil or indomethacin. In conclusion, bradykinin hyperpolarizes MDCK-cells by increasing the apparent potassium conductance. This effect is probably mediated by increase of intracellular calcium activity.

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  1. Physiologisches Institut der Universität Innsbruck, Fritz-Pregl-Strasse 3, A-6010, Innsbruck, Austria

    M. Paulmichl, F. Friedrich & F. Lang

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  1. M. Paulmichl
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  2. F. Friedrich
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  3. F. Lang
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Paulmichl, M., Friedrich, F. & Lang, F. Effects of bradykinin on electrical properties of Madin-Darby canine kidney epithelioid cells. Pflugers Arch. 408, 408–413 (1987). https://doi.org/10.1007/BF00581137

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  • DOI: https://doi.org/10.1007/BF00581137

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