Abstract
In young spontaneously hypertensive rats (SHR) and in normotensive Wistar-Kyoto controls (WKY) several parameters of phosphate and calcium homeostasis were determined. At 6 and 8 weeks, blood analysis revealed a significant hypophosphatemia (p<0.001) in SHR and twice as high plasma calcitonin levels in SHR than in WKY controls. At 8 weeks, 1,25-dihydroxycholecalciferol concentration was 20% higher in SHR (p<0.02) while 25-hydroxycholecalciferol was unaltered (p<0.51). In addition total immunoreactive PTH, iPTH, was slightly increased (p<0.07) but intact PTH (1–84) (p>0.90) was not significantly different from age matched WKY controls. Also at 8 weeks, a slightly reduced serum ionized Ca2+ concentration (p<0.001) with no change in total serum calcium was found in SHR (p>0.39). Balance studies at 6 and 8 weeks of age revealed no significantly different balances for phosphate (F=2.5,p>0.10) and for calcium (F=2.6,p>0.09), although a tendency for slightly more positive balaces existed in SHR when compared to WKY. However, SHR excreted significantly less phosphate in the urine than WKY control (F=0.2,p<0.0009). Bone analysis was performed on femora of SHR and WKY of 6 weeks of age. Femora were significantly shorter in SHR (20.54±0.35 vs. 21.50±0.05 mm in WKY), whereas bone dry weight (127±6 vs. 107±2mg), bone ash weight (79±4 vs. 66±1 mg) and bone volume (0.196±0.007 vs. 0.165±0.004 cm3) were significantly greater in SHR. Calcium content per femur (717±35 vs. 617±11 μmol Ca/femur) and phosphate content per femur (512±23 vs. 447±8 μmol P/femur) were also significantly higher in SHR. It is discussed that the disturbances in phosphate homeostasis may be secondary to the strikingly increased plasma calcitonin levels present in young SHR.
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Bindels, R.J.M., van den Broek, L.A.M., Jongen, M.J.M. et al. Increased plasma calcitonin levels in young spontaneously hypertensive rats: role in disturbed phosphate homeostasis. Pflugers Arch. 408, 395–400 (1987). https://doi.org/10.1007/BF00581135
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DOI: https://doi.org/10.1007/BF00581135