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Pflügers Archiv

, Volume 411, Issue 6, pp 648–653 | Cite as

Fura-2 detected myoplasmic calcium and its correlation with contracture force in skeletal muscle from normal and malignant hyperthermia susceptible pigs

  • Paul A. Iaizzo
  • Werner Klein
  • Frank Lehmann-Horn
Excitable Tissues and Central Nervous Physiology

Abstract

Fura-2 was used to estimate myoplasmic [Ca2+] in intact intercostal muscle fibers from normal and malignant hyperthermia susceptible (MHS) pigs. Small muscle bundles (20–50 fibers) were loaded with the membrane-permeant form of the dye. Resting myoplasmic [Ca2+] were not significantly different in normal and MHS muscles. Halothane produced increases in myoplasmic Ca2+ with associated contractures in MHS muscles, but not in normal muscles. These halothane effects were reversible. Caffeine produced increases in myoplasmic Ca2+ and contractures in both MHS and normal muscles. The threshold concentrations were lower in the MHS muscles. The correlations between myoplasmic [Ca2+] and force in MHS and normal muscles were similar.

Key words

Intercostal muscle fibers Resting [Ca2+Halothane Caffeine Fluorescence 

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Copyright information

© Springer-Verlag 1988

Authors and Affiliations

  • Paul A. Iaizzo
    • 1
  • Werner Klein
    • 1
  • Frank Lehmann-Horn
    • 1
  1. 1.Neurologische Klinik der Technischen Universität MünchenMünchen 80Federal Republic of Germany

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