Pflügers Archiv

, Volume 387, Issue 1, pp 79–81 | Cite as

A new extraction of arginine vasopressin from blood: The use of octadecasilyl-silica

  • Frederick T. LaRochelleJr.
  • William G. North
  • Paul Stern
Transport Processes, Metabolism and Endocrinology; Kidney, Gastrointestinal Tract, and Exocrine Glands Letters and Notes


Small columns packed with octadecasilylsilica were used to extract arginine-vasopressin in femtomole amounts from biological fluids for radioimmunoassay. This method is effective in isolating the peptide from substances in plasma and serum which interfere with its quantitation. Consistent and reproducible results and recoveries >80% were obtained with this procedure. High pressure liquid chromatography and serial dilution of extracted samples confirm the identity of the extracted product.


Arginine vasopressin plasma extraction octadecasilyl-silica 


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.


  1. Bennett, H.P.J., Hudson, A.M., McMartin, C., Purdon, G.E.: Use of octadecasilyl-silica for the extraction and purification of peptides in biological samples. Biochem. J. 168, 9–13 (1977).Google Scholar
  2. Czernichow, P., Patin, A.M., Pomarede, R.: Identification of a macro-molecular form of immuno-reactive arginine vasopressin in human plasma. Program of the 61st Annual Meeting of the Endocrine Society, p. 96 (1979).Google Scholar
  3. Edwards, C.R.W., Chard, T., Kitau, M.J., Forsling, M.L.: The development of a radioimmunoassay and a plasma extraction method for vasopressin. J. Endocrinol. 4, xi-xii (1970).Google Scholar
  4. Johnston, C.I.: Radioimmunoassay for plasma antidiuretic hormone. J. Endocrinol. 52, 69–78 (1972).Google Scholar
  5. Kelley, L., Bennett, H.P.J., Hudson, A.M., McMartin, C., Purdon, G.E.: An efficient method for the extraction of undamaged peptides from various tissues. J. Endocrinol. 77, 24P (1978).Google Scholar
  6. Moore, G., Lutterodt, A., Burford, G., Lederis, K.: A highly specific antiserum for arginine vasopressin. Endocrinology 101, 1421–1435 (1977).Google Scholar
  7. Morton, J.J., Riegger, A.J.G.: A novel extraction method for plasma vasopressin and its application in a radioimmunoassay. J. Endocrinol. 77, 277–278 (1978).Google Scholar
  8. North, W.G., LaRochelle, F.T., Jr., Haldar, J., Sawyer, W.H., Valtin, H.: Characterization of an antiserum used in a radioimmunoassay for arginine-vasopressin: Implications for reference standards. Endocrinology 103, 1976–1984 (1978).Google Scholar
  9. O'Hare, M.J., Nice, E.C.: Hydrophobic high-performance liquid chromatography polypeptides and proteins on alkylsilane-bonded silica. J. Chromatography 171, 209–226 (1979).Google Scholar
  10. Robertson, G.L., Klein, L.A., Roth, J., Gorden, P.: Immunoassay of plasma vasopressin in man. Proc. Nat. Acad. Sci. (USA) 66, 1298–1305 (1970).Google Scholar
  11. Robertson, G.L., Mahr, E.A., Athar, S., Sinha, T.: Development and clinical application of a new method for the radioimmunoassay of arginine vasopressin in human plasma. J.C.I. 52, 2340–2352 (1973).Google Scholar
  12. Robinson, A.G., Frantz, A.G.: Radioimmunoassay of Posterior Pituitary Peptides: A Review. Metabolism 22, 1047–1057 (1973).Google Scholar
  13. Robinson, I.C.A.F., Walker, J.M.: Extraction of small amounts of oxytocin from biological fluids by means of agarose-bound neurophysin J. Endocrinol. 80, 191–202 (1979).Google Scholar
  14. Rodbard, D. and Lewald, J.E.: Computer analysis of radioligand assays and radioimmunoassay data. Karolinski Symposia, Research Methods in Reproductive Endocrinology, Geneva March 1970. Acta Endocrinol. 64, Suppl. 147, 79–103 (1970).Google Scholar
  15. Skowsky, W.R., Rosenbloom, A.A., Fisher, D.A.: Radioimmunoassay measurement of arginine vasopressin in serum: Development and application J. Clin. Endocrinol. 38, 278–287 (1974).Google Scholar
  16. Thomas, T.H., Lee, M.R.: The specificity of antisera for the radioimmunoassay of argininevasopressin in human plasma and urine during water loading and dehydration. Clin. Sci. Mol. Med. 51, 525–536 (1976).Google Scholar

Copyright information

© Springer-Verlag 1980

Authors and Affiliations

  • Frederick T. LaRochelleJr.
    • 1
  • William G. North
    • 1
  • Paul Stern
    • 1
  1. 1.Departments of Physiology and Maternal and Child HealthDartmouth Medical SchoolHanoverUSA

Personalised recommendations