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Pflügers Archiv

, Volume 387, Issue 1, pp 79–81 | Cite as

A new extraction of arginine vasopressin from blood: The use of octadecasilyl-silica

  • Frederick T. LaRochelleJr.
  • William G. North
  • Paul Stern
Transport Processes, Metabolism and Endocrinology; Kidney, Gastrointestinal Tract, and Exocrine Glands Letters and Notes

Summary

Small columns packed with octadecasilylsilica were used to extract arginine-vasopressin in femtomole amounts from biological fluids for radioimmunoassay. This method is effective in isolating the peptide from substances in plasma and serum which interfere with its quantitation. Consistent and reproducible results and recoveries >80% were obtained with this procedure. High pressure liquid chromatography and serial dilution of extracted samples confirm the identity of the extracted product.

Keywords

Arginine vasopressin plasma extraction octadecasilyl-silica 

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References

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Copyright information

© Springer-Verlag 1980

Authors and Affiliations

  • Frederick T. LaRochelleJr.
    • 1
  • William G. North
    • 1
  • Paul Stern
    • 1
  1. 1.Departments of Physiology and Maternal and Child HealthDartmouth Medical SchoolHanoverUSA

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