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Pflügers Archiv

, Volume 407, Issue 2, pp 145–152 | Cite as

Impairment of Na+ transport across frog skin by Tl+: Effects on turnover, area density and saturation kinetics of apical Na+ channels

  • W. Zeiske
  • W. Van Driessche
Transport Processes, Metabolism and Endocrinology; Kidney, Gastrointestinal Tract, and Exocrine Glands

Abstract

  1. 1.

    Na+ transport across abdominal skin of the frogs,Rana temporaria andRana esculenta was followed by measuring Na+-dependent short-circuit, current (INa) kinetics andINa fluctuations induced by triamterene, a diuretic.

     
  2. 2.

    Exposure of the skin to serosal Tl+ led to a pronounced and irreversible drop inINa andINa-blocker noise.

     
  3. 3.

    At low serosal Tl+ concentrations, we observed mainly a decrease in the apparent Michaelis constant forINa saturation while, at larger [Tl+], the maximalINa dropped irreversibly. Tl+ acts even when serosal Tl+ “transporters” like the Na+−K+ pump, or the K+ channel are nonfunctional.

     
  4. 4.

    The rate constants for the triamterene/Na+ channel reaction were unchanged after Tl+ whereas the relaxation noise from channel blockage decreased in amplitude. Noise analysis in terms of a two-state blocking model suggested that Tl+ poisoning results in a small decrease in singlechannel current through apical Na+ pathways, as well as in a drastic and irreversible drop in channel density.

     
  5. 5.

    The impairment of Na+ transport by Tl+ can be attributed to the above cited concerted events at the level of the apical membrane.

     

Key words

Frog skin Na+ transport Apical membrane Noise analysis Thallium 

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Copyright information

© Springer-Verlag 1986

Authors and Affiliations

  • W. Zeiske
    • 1
  • W. Van Driessche
    • 2
  1. 1.Institut für Tierphysiologie und Angewandte ZoologieFreie Universität BerlinBerlin 41
  2. 2.Laboratorium voor FysiologieLeuvenBelgium

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