Abstract
The template-engaged RNA polymerase II was assayed in the nuclei purified from the cerebral hemisphere of 3-, 14- and 30-day old rats. Its activity is the highest at 3-day and declines as development proceeds. Lower transcription at 30-day may either be due to a decrease in the active fraction of chromatin or to a decrease in the amount of RNA polymerase II that is active towards endogenous template, or both. The activity of RNA polymerase I (active in low salt) is also maximal at 3-day and declines as development proceeds. (ADP)ribosylation of chromatin depresses RNA synthesis. This may be due to inactivation of RNA polymerase itself by protein poly(ADP)ribosylation.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
Refences
Yu F. L., 1974. Nature 251: 344–346.
Yu F. L., 1975. Biochim. Biophys. Acta 395: 329–336.
Reeder R. H. & Roeder R. G., 1972. J. Molec. Biol. 67: 433–441.
Weinmann R. & Roeder R. G., 1974. Proc. Natl. Acad. Sci. U.S.A. 71: 1790–1794.
TataJ. R. & Baker B., 1978. J. Mol. Biol 118: 249–272.
Berger N. A., Petzold S. J. & Berger S. J., 1979. Biochim. Biophys. Acta 564: 90–104.
Gray D. A., Durkacz B. W. & Shall S., 1981. FEBS Lett 121: 173–177.
Burzio L. & Koide S. S., 1970. Biochem. Biophys. Res. Commun. 40: 1013–1020.
Yoshihara K. & Koide S. S., 1973. FEBS Lett. 35: 262–264.
Muller W. E. G., Tetsuka A., Nusser I., Obermeir J., Rhode H. J. & Zahn R. K., 1974. Nucleic Acids Res. 1: 1317–1327.
Muller W. E. G. & Zahn R. K., 1976. Mol. Cell Biochem. 12: 147–159.
Caplan A. I. & Rosenberg N. J., 1975. Proc. Natl. Acad. Sci. U.S.A. 72: 1852–1857.
Supakar P. C. & Kanungo M. S., 1982. Biochem. Int. 5: 381–388.
Blobel G. & Potter V., 1966. Science 154: 1662–1665.
Panyim S., Bilek D. & Chalkley R., 1971. J. Biol. Chem. 246: 4206–4215.
Rickwood D., Riches P. G. & McGilvray A. I., 1973. Biochim. Biophys. Acta 299: 162–171.
Schwartz L. B., Sklar V. E. F., Jaehning J. A., Weenman R. & Roeder R. G., 1974. J. Biol. Chem. 249: 5889–5897.
Supakar P. C. & Kanungo M. S., 1984. Mol. Biol. Rep. 9: 253–257.
Sims J. L., Sikorski G. W., Catino D. M., Berger S. J. & Berger N. A., 1982. Biochemistry 21: 1813–1821.
Purnell N. R. & Whish W. J. D., 1980. Biochem. J. 135: 775–777.
Okayama H. & Hayaishi O., 1978. Biochem. Biophys. Res. Commun. 84: 755–762.
Ballal N. R. & Busch H., 1973. Cancer Res. 33: 2737–2743.
Jump D. B. & Smulson M., 1980. Biochemistry 19: 1024–1030.
Ogata N., Kawaichi M., Ueda K. & Hayaishi O., 1980. Biochem. Int. 1: 229–236.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Das, B.R., Kanungo, M.S. Effect ofin vitro (ADP)ribosylation on transcription of the chromatin of the brain of developing rats. Mol Biol Rep 12, 43–47 (1987). https://doi.org/10.1007/BF00580649
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF00580649