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Reaction of serum albumin with dimethyl sulfoxide according to PMR spectra of frozen aqueous solutions

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Abstract

The reaction of human serum albumin (HSA) with dimethyl sulfoxide (DMSO) was studied by the PMR method according to the intensity of the proton signal of non-freezable water in the temperature range of 230–273°K. It was shown that the concentration of DMSO in the non-frozen liquid phase is much higher than should be expected for the simple eutectic. This is attributable to the binding of N molecules of DMSO with a molecule of HSA (at 270°K, N = 7–10). It was found that in the presence of DMSO, the amount of non-freezable water markedly increased, which is possibly due to a reaction between DMSO and proton-active centers of the protein through the intermediate water molecules.

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Authors and Affiliations

  1. L. V. Pisarzhevskii Institute of Physical Chemisry, Academy of Sciences of the Ukrainian SSR, Kiev

    V. K. Pogorelyi, V. V. Turov, A. V. Turov & V. N. Barvinchenko

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  1. V. K. Pogorelyi
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  2. V. V. Turov
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  3. A. V. Turov
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  4. V. N. Barvinchenko
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Translated from Teoreticheskaya i Ékperimental'naya Khimiya, Vol. 25, No. 1, pp. 104–108, January–February, 1989.

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Pogorelyi, V.K., Turov, V.V., Turov, A.V. et al. Reaction of serum albumin with dimethyl sulfoxide according to PMR spectra of frozen aqueous solutions. Theor Exp Chem 25, 93–97 (1989). https://doi.org/10.1007/BF00580307

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  • DOI: https://doi.org/10.1007/BF00580307

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