Rheumatology International

, Volume 14, Issue 4, pp 135–138 | Cite as

Serum IgM rheumatoid factor by enzyme-linked immunosorbent assay (ELISA) delineates a subset of patients with deforming joint disease in seronegative juvenile rheumatoid arthritis

  • A. Aggarwal
  • S. Dabadghao
  • S. Naik
  • R. Misra
Original Article


Using human IgG as an antigen in an enzymelinked immunosorbent assay (ELISA), we looked for the presence of IgM rheumatoid factor (RF) in the sera of 74 children with juvenile rheumatoid arthritis (JRA). Nine children had RF detectable by both latex agglutination and ELISA. Forty-five percent (26 of 65) of the children who were seronegative by latex agglutination were found to be positive for IgM RF by ELISA. The prevalence of IgM RF was higher in patients with polyarticular onset disease (57.4%) than in those with pauciarticular onset (38.5%) or systemic onset (27.2%) disease. The prevalence of RF was higher in sera from patients with deforming joint disease than those without deformities (P<0.01).

Key words

JCA Autoantibodies Disease severity 


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  1. 1.
    Lang BA, Shore A (1990) A review of current concepts on the pathogenesis of juvenile rheumatoid arthritis. J Rheumatol 17 (Suppl 21):1–15Google Scholar
  2. 2.
    Haynes DC, Gershwin ME, Robbins DL, Miller JJ, Cosca D (1986) Autoantibody profile in juvenile arthritis. J Rheumatol 13:358–363Google Scholar
  3. 3.
    Moore TL, Dorner RW, Alexander RL, Osborn TG (1988) Enzyme linked immunosorbent assay for the detection of hidden 19S IgM rheumatoid factor in juvenile rheumatoid arthritis. J Rheumatol 15:87–90Google Scholar
  4. 4.
    Walker SM, Shaham B, McCurdy DK, Wietting H, Arora YK, Hanson V, Bernstein B (1990) Prevalence and concentration of IgM rheumatoid factor in polyarticular onset disease as compared to systemic or pauciarticular onset disease in active juvenile rheumatoid arthritis as measured by ELISA. J Rheumatol 17:936–940Google Scholar
  5. 5.
    Jarvis JN, Pousak T, Krenz M (1992) Detection of IgM rheumatoid factor by enzyme-linked immunosorbent assay in children with juvenile rheumatoid arthritis: correlation with articular disease and laboratory abnormalities. Pediatrics 90:945–949Google Scholar
  6. 6.
    Brewer EJ Jr, Bass J, Cassidy JT (1977) Current proposed revision of JRA criteria. Arthritis Rheum 20:195–199Google Scholar
  7. 7.
    Faith A, Pontesilli O, Unger A (1982) ELISA assays for IgM and IgG rheumatoid factors. J Immunol Methods 55:169–177Google Scholar
  8. 8.
    Wernik R, Lo Spalluto JJ, Fink CW, Ziff M (1981) Serum IgG and IgM rheumatoid factor by solid phase radioimmunoassay. Arthritis Rheum 24:1501–1511Google Scholar
  9. 9.
    Arnett FC, Edworthy SM, Bloch DA, McShone DJ, Fries JF, Cooper NS, Liang MH, Luthra HS, Medsger TA Jr, Mitchell DM, Neustadt DH, Pineals RS, Schaller JG, Sharp JT, Wilder RL, Hunder GG (1988) The American Rheumatism Association 1987 revised criteria for the classification of rheumatoid arthritis. Arthritis Rheum 31:315–324Google Scholar
  10. 10.
    Harris ED (1990) Rheumatoid arthritis: pathophysiology and implications for therapy. N Engl J Med 322:1277–1289Google Scholar
  11. 11.
    Magsaam J, Ferjencik P, Temples M, Dippell J (1987) A new method for the detection of hidden IgM rheumatoid factor in patients with juvenile rheumatoid arthritis. J Rheumatol 14:964–967Google Scholar
  12. 12.
    Lawrence JM, Moore TL, Osborn TG, Nesher G, Madson KL, Kinsella MB (1993) Autoantibody studies in juvenile rheumatoid arthritis. Semin Arthritis Rheum 22:265–274Google Scholar

Copyright information

© Springer-Verlag 1994

Authors and Affiliations

  • A. Aggarwal
    • 1
  • S. Dabadghao
    • 1
  • S. Naik
    • 1
  • R. Misra
    • 1
  1. 1.Department of ImmunologySanjay Gandhi Postgraduate Institute of Medical SciencesLucknowIndia

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