Chemistry of Natural Compounds

, Volume 21, Issue 4, pp 517–520 | Cite as

Determination of the cystine and cysteine contents of proteins with the aid of an amino acid analyzer

  • N. I. Koryakina
  • T. P. Kopitsya
  • G. F. Kasymova
  • V. K. Burichenko
Article

Abstract

A method is proposed which permits the determination of the amounts of cystine an cysteine in the form pf systeic acid in various proteins of animal and plant origin — even in those in which, on the analysis of standard hydrolysates, the peaks of cysteine are not detected because of their low concentration.

Keywords

Cysteine Cystine Aspartic Acid Amino Acid Analysis Cysteic Acid 

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Literature cited

  1. 1.
    C. H. W. Hirs, Methods Enzymol.,11, 197 (1967).CrossRefGoogle Scholar
  2. 2.
    Methods for the Protein and Amino Acid Analysis of Plants. Methodological Instructions [in Russian], Leningrad (1973), p. 20.Google Scholar
  3. 3.
    R. J. Spenser and F. Wold, Anal. Biochem.,32, No. 1, 185 (1969).CrossRefGoogle Scholar
  4. 4.
    I. Isenberg, Annu. Rev. Biochem.,48, 159 (1979).CrossRefGoogle Scholar
  5. 5.
    Z. Deyl, K. Macek, and J. Janak, Liquid Column Chromatography, Elsevier, Amsterdam (1975).Google Scholar
  6. 6.
    G. F. Kasymova, T. P. Kopitsya, N. I. Koryakina, and V. K. Burichenko, Khim. Prir. Soedin., 470 (1981).Google Scholar

Copyright information

© Plenum Publishing Corporation 1986

Authors and Affiliations

  • N. I. Koryakina
  • T. P. Kopitsya
  • G. F. Kasymova
  • V. K. Burichenko

There are no affiliations available

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