Relation between the increase in activity of some hydrolases and genomic activation during regeneration

  • M. Fontés
Article

Summary

One of the most important biochemical aspects of regeneration, in the early stages, after amputation, is the increase in acid hydrolase activities. During regeneration, 24h after amputation of the anterior part of the body of the polychaeteOwenia fusiformis, we have observed the following events:
  1. 1.

    The increase of3H-Uridine incorporation in microsomal and nuclear fractions.

     
  2. 2.

    After one hour's labelling the major part of the radioactivity is localized on polysomal structures.

     
  3. 3.

    By use of specific drugs (cycloheximide and actinomycin D at various concentrations) we have attempted demonstrating the relation between the activation of synthesis of RNAs (probably mRNA) and the new synthesis of acid hydrolase molecules.

     

Keywords

Developmental Biology Major Part Anterior Part Cycloheximide Specific Drug 

Abbreviations

mRNA

messenger RNA

TKM

Tris-HCl buffer 0.2 N+KCl 0.025 M+MgCL2 0.005 M pH 7.6

PVS

Polyvinyl sulfate

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References

  1. Bergmeyer, H. U.: Methods of enzymatic analysis. NewYork-London: Academic Press1963 Google Scholar
  2. Buongiorno-Nardelli, M., Thouveny, Y.: Données histochimiques et enzymologiques sur la régénération antérieure de l'Annelide PolycheteOwenia fusiformis. Bull. biol. France Belg.C4, 488–516 (1966)Google Scholar
  3. De Duve, C., Pressman, B. C., Gianetto, R., Wattiaux, R., Appelmans. F.: Tissue fractionation studies 6 — Intracellular distribution patterns of enzymes in rat liver tissue. Biochem. J.60, 604–617 (1955)Google Scholar
  4. Fontés, M., Thouveny, Y.: Inhibition de la régénération antérieure de l'AnnelideOwenia fusiformis par l'actinomycine D et le thiophénicol en rapport avec la synthèse de RNA. C. R. Acad. Sci. (Paris)273, 1873–1876 (1971a)Google Scholar
  5. Fontés, M., Thouveny, Y.: Activité de la ribonucléase acide et de la ribonucléase alcaline dans la régénération antérieure de l'AnnelideOwenia fusiformis. C. R. Acad. Sci. (Paris)273, 2609–2612 (1971b)Google Scholar
  6. Pennman, S., Vesco, C., Pennman, M.: Localisation and kinetics of nuclear heterodisperse RNA, cytoplasmic heterodisperse RNA and polyribosome associated messenger RNA in HeLa cells. J. molec. Biol.34, 63 (1968)Google Scholar
  7. Perry, R. P.: Selective effects of actinomycin D on RNA synthesis. Exp. Cell. Res.72 400–106 (1963)Google Scholar
  8. Roberts, W. K., Newman, J.: Use of low concentrations of actinomycin D in the study of RNA synthesis in Ehrlich Ascites cells. J. molec. Biol.20, 63 (1966)Google Scholar
  9. Schimke, R., Doyle, P.: Control of enzyme levels in animal tissus. Ann. Rev. Biochem.39, 930–968 (1970)Google Scholar
  10. Spirin, A. S.: On „masked“ forms of messenger RNA in early embryogenesis and in other differentiating systems. Curr. Top. Develop. Biol.1, 1 (1966)Google Scholar
  11. Thouveny, Y.: Les systems histogenétiques et la dédifféreciation cellulaire dans la morphogenèse des Annelides Polychètes. Arch. Zool. exp. gen.108, (3), 347–386 (1967)Google Scholar
  12. Urbani, E.: In: Regeneration in animals and related problems, eds. V. Kiortsis and H. A. L. Trampusch, p. 39. Amsterdam: North-Holland Publ. 1965Google Scholar

Copyright information

© Springer-Verlag 1974

Authors and Affiliations

  • M. Fontés
    • 1
  1. 1.Laboratoire de Morphogenèse AnimaleCentre Universitaire de LuminyFrance

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