, Volume 68, Issue 3, pp 179–181 | Cite as

Immunization of mice with an avirulent pseudohyphal form of Cryptococcus neoformans

  • Robert A. Fromtling
  • Rebecca Blackstock
  • Nancy K. Hall
  • Glenn S. Bulmer


Mice were immunized with a viable, avirulent strain ofCryptococcus neoformans. Lymphocyte blastogenic assays showed a 10-fold increase in reactivity of sensitized spleen cells, and histopathologic examination revealed marked splenic hyperplasia. Thirty-two days after intravenous inoculation with a virulent strain ofC. neoformans, none of the control animals survived whereas 60 percent of the immunized mice were alive with no clinical evidence of disease. This animal model shows that protective immunity can be established, and once developed, provide a better model for the study of important aspects of immunity in fungal disease.


Animal Model Control Animal Clinical Evidence Histopathologic Examination Virulent Strain 
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Copyright information

© Dr. W. Junk bv - Publishers 1979

Authors and Affiliations

  • Robert A. Fromtling
    • 1
  • Rebecca Blackstock
    • 2
  • Nancy K. Hall
    • 3
  • Glenn S. Bulmer
    • 1
  1. 1.Department of Microbiology and ImmunologyThe University of Oklahoma Health Sciences Center (OUHSC)Oklahoma CityUSA
  2. 2.Department of PediatricsOUHSCUSA
  3. 3.Department of PathologyOUHSCUSA

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