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Applied Microbiology and Biotechnology

, Volume 45, Issue 4, pp 454–457 | Cite as

Large-scale perfusion culture process for suspended mammalian cells that uses a centrifuge with multiple settling zones

  • H. Takamatsu
  • K. Hamamoto
  • K. Ishimura
  • S. Yokoyama
  • M. Tokashiki
Biotechnology Short Contribution

Abstract

A high-cell-density perfusion culture process, using a novel centrifuge, was developed. The centrifuge has spiral multiple settling zones to separate cells from culture medium. Because of the multiple zones, the separation area can be efficiently increased without enlarging the diameter of the centrifuge. The centrifuge used in this study had a separation capacity of 2600 ml culture medium min−1 at 100g of the centrifugal force. A new cell separation and withdrawal method was also developed. The cells separated in the centrifuge can be withdrawn easily from the centrifuge with no cell clogging by feeding a liquid carrier such as a perfluorocarbon into the centrifuge and pushing the cells out with the liquid carrier. By this culture process, monoclonal antibodies were produced with mouse-human hybridoma X87X at a cell density of about 8 × 106 cells ml−1 for 25 days. This centrifuge culture shows promise as a large-scale perfusion culture process.

Keywords

Centrifuge Monoclonal Antibody Cell Density Mammalian Cell Settling 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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References

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Copyright information

© Springer-Verlag 1996

Authors and Affiliations

  • H. Takamatsu
    • 1
  • K. Hamamoto
    • 1
  • K. Ishimura
    • 1
  • S. Yokoyama
    • 1
    • 2
  • M. Tokashiki
    • 1
  1. 1.Biotechnology Research LaboratoriesTokyo Research Center, Teijin LimitedHino TokyoJapan
  2. 2.Research Planning DepartmentTeijin LimitedChiyoda-ku TokyoJapan

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